27,462 results
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Plasmid#205619PurposeExpression of BCR-ABL oncogene in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBCR-ABL oncogene, p210 isoform b3a2
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TEK-V5/HIS
Plasmid#205611PurposeExpression of human TEK receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTEK receptor tyrosine kinase (TEK Human)
TagsV5-HisExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA351-Cpf1
Plasmid#192361PurposeCRISPR-based vector for genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas12a, gRNA scaffold
UseCRISPRAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFFLuc-IVT
Plasmid#205147PurposeIVT template of Firefly luciferase stop codon between the ORF and MCS and polyADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFirefly luciferase
UseLuciferase; In-vitro transcriptionAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-mCherry
Plasmid#201474PurposeIndirect microscopy/imaging of V5-tagged proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNbV5-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFR-mApple
Plasmid#203773PurposeNumber and brightness analysis of EGFR oligomerizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFR (EGFR Human)
TagsmAppleExpressionMammalianAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-wtFAKSPARK2
Plasmid#195263Purposeexpress wtFAKSPARK2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFAKSPARK2 sensor
ExpressionMammalianPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax-SpRY
Plasmid#195279PurposeAllows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
ExpressionMammalianPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-FGFR4-V5/HIS
Plasmid#201109Purposeexpression of human FGFR4 receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-FGFR3c-V5/HIS
Plasmid#201108Purposeexpression of human FGFR3 receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL5069 (pDonor(AAVS1),CRISPR_Pse)
Plasmid#200904PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
ExpressionMammalianAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRP4-Flag/pCDNA3.1
Plasmid#197291PurposePlasmid expressing an antigen targeting LRP4 (extracellular)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLRP4 (Lrp4 Mouse)
TagsFLAGExpressionMammalianAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpRYc
Plasmid#175575PurposeExpresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpRYc
TagsEGFPExpressionMammalianMutationPID of SpRY grafted onto the N-terminal domain of…PromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mGreenLantern
Plasmid#197928PurposeMonomer expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmGreenLantern
ExpressionMammalianMutationnoneAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Membrane mNeonGreen
Plasmid#198057PurposePlasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLCK membrane mNeonGreen (LCK Human, A.victoria)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmNeonGreenMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196078Purpose(Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
ExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLexM-SLC7A11_GFP
Plasmid#197995PurposeExpresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSLC7A11 (SLC7A11 Human)
TagsFLAG tag and TEV-GFP-His8ExpressionMammalianPromoterCMVAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet3_N1
Plasmid#189778PurposeConstruct for testing oligimerization of mScarlet3 in mammalian cells by OSER assayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCyterm-mScarlet3
ExpressionMammalianPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)
Plasmid#197508PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…PromoterCMV and T7Available sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Td-CBEmax
Plasmid#196600PurposeMammalian expression of Td-CBEmaxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
Tags6*HisExpressionMammalianMutationTadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)Available sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by