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30,870 results

Showing: 1261 - 1280 of 30870 results

  7. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65771-plasmid-map-sequence-id-112653

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  16. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140002-plasmid-map-sequence-id-275516

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  17. pCS2+IMS-HyPer7

    Plasmid
    #136469
    Purpose
    Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Publication
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPer7
    Tags
    SMAC/DIABLO
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136469-plasmid-map-sequence-id-271548

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  18. mEmerald-Sec61b-C1

    Plasmid
    #90992
    Purpose
    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells
    Depositor
    Jennifer Lippincott-Schwartz
    Publication
    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEmerald-Sec61b (SEC61B Human, Synthetic)
    Tags
    mEmerald (green fluorescence)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90992-plasmid-map-sequence-id-179977

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  19. pICE-HA-MRE11-WT

    Plasmid
    #82033
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82033-plasmid-map-sequence-id-490398

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Showing: 1261 - 1280 of 30870 results