31,786 results
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pMAL-c2X_MBP-CLmN-H6
Plasmid#234487PurposeMaltose binding protein N-terminally fused to His-tagged CLmN split inteinDepositorInsertN-terminal Fragment of the CLm Split Intein
TagsHexahistidine Tag and Maltose Binding Protein (MB…ExpressionBacterialMutationT69K, F75H, M118N within the N-terminal fragment …Available SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
14x PP7 with loxP-kanMX-loxP
Plasmid#189939PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)DepositorInsert14x PP7 with loxP-kanMX-loxP
TagskanMXExpressionBacterialAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p324_DG
Plasmid#234690PurposeGolden gate domesticated pET28b with TEV-His6DepositorTypeEmpty backboneTagsTEV-His6ExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
ExpressionBacterialAvailable SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 NusA TEV LIC cloning vector (1N)
Plasmid#29657DepositorTypeEmpty backboneTags6xHis, NusA, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMG024 MBP-GSK3β-HA-His, GST_λPPase
Plasmid#196182PurposeCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-HisDepositorAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-GGA3-VHS-GAT
Plasmid#173719PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.DepositorAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-His
Plasmid#119755PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.DepositorTypeEmpty backboneTags10xHis and GSTExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEERM4 Cm
Plasmid#64026PurposePlasmid for integration into neutral site 2 of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPnrsBAvailable SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
TagsHis tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-DENV3-NS5FL
Plasmid#86013PurposeExpression of dengue virus 3 NS5 proteinDepositorInsertDENV3-NS5FL
Tagshexa-histidine tagExpressionBacterialMutationamino acid residues 6 to 895 of DENV3 NS5PromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
MYST3
Plasmid#25181PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMYST3_13 (KAT6A Human)
TagsHisExpressionBacterialMutationcontains catalytic region ( residues 497-780).Available SinceOct. 25, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterrrnB P1Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pEERM3 Km
Plasmid#64025PurposePlasmid for integration into neutral site of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPnrsBAvailable SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28HIS-hAPE1
Plasmid#70757Purposebacterial expression of human APE1DepositorAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only
