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pEN-L1-K2-L2

Plasmid

#80684

Purpose

Gateway Entry vector containing a temperature-sensitive mouse DHFR

Depositor

Nico Dissmeyer

Article

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Inserts

DHFR (Dhfr Mouse)
DHFR (Dhfr Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Mutation

mouse DHFR with N-terminal Ubiquitin

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRF-LmrA

Plasmid

#49364

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

LmrA repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET32a-hBTC

Plasmid

#199230

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Betacellulin (BTC Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-HIS-sfGFP-DocI

Plasmid

#58708

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

sfGFP-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427V

Plasmid

#81044

Purpose

mammalian expression of LOV2 I427V

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427V

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427V

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNTD

Plasmid

#157869

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaNTD

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-UTPase

Plasmid

#12667

Depositor

SeWon Suh

Article

Han et al Acta Crystallogr D Biol Crystallogr. 200

Insert

dUTP pyrophosphatase (DUT1 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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