30,416 results

1. 5 pCOLI_A_STREP

   Plasmid

   #160147

   Purpose

   N-term 2xStrepII 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   2xStrepII

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet1_CoiC_CoiSA

   Plasmid

   #208762

   Purpose

   Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiC
   CoiSA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   single nucleotide insertion (C70) at RBS of CoiC

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMG-RIS

   Plasmid

   #225132

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pREDCas9

   Plasmid

   #71541

   Purpose

   Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   lambda red genes
   plasmid curing system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. 37 pCOLI_K_STREP

   Plasmid

   #160263

   Purpose

   N-term 2xStrepII 3C cleavable Kan resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   2xStrepII

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACyR1

   Plasmid

   #172565

   Purpose

   Inducible gene expression, pACYC origin, CymRAM repressor, PCymRC promoter, mRFP, Chloramphenicol Resistance Marker.

   Depositor

   Christopher Reisch

   Insert

   CymRAM-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCymRC

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHyperTEV60

   Plasmid

   #228496

   Purpose

   10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   TEV Protease
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQLink-GST-4xCTD

   Plasmid

   #138471

   Purpose

   Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   Rpb1 (POLR2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   4x CTD repeat

   Promoter

   T5

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEvol-MmKlacRS1

   Plasmid

   #232157

   Purpose

   Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   KlacRS1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLuc-N-HiBiT

    Plasmid

    #207117

    Purpose

    Bacterially expressed HiBiT-FLuc for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    luciferin 4-monooxygenase
    

    Use

    Tags

    HiBiT Tag and His-tag with thrombin site

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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