30,738 results

1. pAKgfplux1

   Plasmid

   #14083

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Expression

   Bacterial

   Available Since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV_pTp66p51

   Plasmid

   #78233

   Purpose

   Expresses HIV reverse transcriptase

   Depositor

   Christopher Voigt

   Article

   Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

   Inserts

   p66
   p51
   LacI
   

   Expression

   Bacterial

   Promoter

   Const. Promoter (From DNA2.0 Vec) and pTac Minima…

   Available Since

   Aug. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNHEJ-recX-sacB

   Plasmid

   #158718

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Expression

   Bacterial

   Promoter

   Pmyc

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTET GFP10-FRB/FKBP-GFP11

   Plasmid

   #182497

   Purpose

   Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Inserts

   FRB
   FKBP (Fkbp1a Mouse, Synthetic)
   

   Tags

   split GFP10 tripartite and split GFP11 tripartite

   Expression

   Bacterial

   Promoter

   TET

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSUN2

   Plasmid

   #188059

   Purpose

   Bacterial expression plasmid for human NSUN2; for activity testing

   Depositor

   Tanja Schirmeister

   Article

   Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

   Insert

   NSUN2 (NSUN2 Human)
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRS-LacI-GFP

   Plasmid

   #220198

   Purpose

   mini-Tn7 GFP expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   monomeric superfolder green fluorescent protein
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   LacI/pLac

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOSV00170

   Plasmid

   #222934

   Purpose

   Constitutive GFP expression for Bacillus subtilis

   Depositor

   Ophelia Venturelli

   Article

   Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

   Insert

   GFP(Sp)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Phyperspank

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

   Plasmid

   #218508

   Purpose

   integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mNeongreen
   

   Tags

   modified ssrA degradation tag

   Expression

   Bacterial

   Mutation

   deletion of MCS containing Plac_lacZa, introducti…

   Promoter

   P23

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-hEGF

   Plasmid

   #199231

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Epidermal growth factor, partial (EGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHN781

    Plasmid

    #178828

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A (AR Human)
    

    Tags

    His8, TEV

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYCDuet-1:ChrA

    Plasmid

    #225079

    Purpose

    Expresses precursor peptide ChrA in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBzCas13b-gRNA-NT

    Plasmid

    #164861

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-NT
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBzCas13b-gRNA-1

    Plasmid

    #164858

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-1
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB076i7

    Plasmid

    #79201

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM4Z-Fluc

    Plasmid

    #183474

    Purpose

    This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST-KT

    Plasmid

    #44561

    Purpose

    for ectopic inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Promoter

    UV15 promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDM069

    Plasmid

    #216821

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold2
    

    Tags

    RIPGLIN and VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPgiCas13b-gRNA-NT

    Plasmid

    #184835

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPlacPbuCas13b-gRNA-NT

    Plasmid

    #184838

    Purpose

    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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