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pECJ3B

Plasmid

#75466

Purpose

Toggle switch plasmid containing lacI-pdt3B

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

lacI-pdt3B

Expression

Bacterial

Promoter

pLtetO

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMTB2-cytoBirA-2A-mCherry_Ras

Plasmid

#80066

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Beta-actin2

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescript JTV-1

Plasmid

#16473

Depositor

Bert Vogelstein

Article

Nicolaides et al Genomics. 1995 Sep 20. 29(2):329-34.

Insert

JTV-1 (AIMP2 Human)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pBW115lac-hrpR

Plasmid

#61434

Purpose

pAPT110 encoding rbsH-hrpR-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbsH-hrpR-ter

Expression

Bacterial

Promoter

pLac

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)

Plasmid

#48291

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Sumo

Expression

Bacterial

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Stat1-SH2

Plasmid

#46511

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat1 (STAT1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 488-683

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi_PC-σ

Plasmid

#214040

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-Pci-PC-σ

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET21(+)-His6-Ubc13

Plasmid

#212715

Purpose

Bacterial expression of His6-Ubc13

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAllet

Plasmid

#202560

Purpose

E. coli cloning vector with large MCS (50+ unique restriction sites)

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC59-Halo

Plasmid

#175327

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC59 (CCDC59 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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