30,738 results

1. pUC19 PKC delta

   Plasmid

   #8427

   Depositor

   Frederic Mushinski

   Article

   Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

   Insert

   Mouse Protein Kinase C delta (Prkcd Mouse)
   

   Expression

   Bacterial

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6p-2-EWSR1

   Plasmid

   #46384

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

   Plasmid

   #136627

   Purpose

   Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-LAMA-R59
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLAG_OriM

   Plasmid

   #110097

   Purpose

   Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3xFLAG

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. HTATIP (2OU2)

   Plasmid

   #33338

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HTATIP (KAT5 Human)
   

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pIB170

   Plasmid

   #90193

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Tags

   None

   Expression

   Bacterial

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-scFv-T

   Plasmid

   #67843

   Purpose

   pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

   Depositor

   Joanna Bereta

   Article

   Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

   Insert

   antibody 1D8 light kappa chain
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMVV104

   Plasmid

   #215310

   Purpose

   Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   PAraBAD

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

   Plasmid

   #128164

   Purpose

   UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).

   Depositor

   Marcus Noyes

   Article

   Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

   Insert

   10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
   

   Use

   Synthetic Biology

   Tags

   FLAG and zif268

   Expression

   Bacterial

   Promoter

   UV5

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt, Kathleen Burns

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ydf NanoLuc

    Plasmid

    #225656

    Purpose

    Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NanoLuciferase
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Promoter

    PydfJ115

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL NarL YdfI Flag

    Plasmid

    #225650

    Purpose

    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NarL-YdfI (narL Synthetic)
    

    Use

    Cell free expression

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    NarL with native DNA-binding domain replaced with…

    Promoter

    T7

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL NarX Myc

    Plasmid

    #225651

    Purpose

    Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NarX (narX E. coli)
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLyGo-Ec-1

    Plasmid

    #163127

    Purpose

    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Ubiquitin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTP1005

    Plasmid

    #104160

    Purpose

    Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUltra-sY

    Plasmid

    #82417

    Purpose

    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Methanococcus jannaschii aaRS for sulfotyrosine
    

    Expression

    Bacterial

    Mutation

    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

    Promoter

    tacI

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. NP-11-64-1

    Plasmid

    #234359

    Purpose

    Expressing rubisco and prk in Δrpi

    Depositor

    David Savage

    Article

    Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    R.rubrum rubisco
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPbcas13b

    Plasmid

    #89906

    Purpose

    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SRPK1 (SRPK1A-c100)

    Plasmid

    #98246

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SRPK1A (SRPK1 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule2 - Mj Acd A9

    Plasmid

    #197652

    Purpose

    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

    Depositor

    Ryan Mehl

    Article

    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

    Inserts

    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32A, L65D, F108L, Q109E, D158S, L162T

    Promoter

    GlnS and lpp

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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