31,455 results
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Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonExpressionBacterialMutationTGTTGATG --> TGTTGGAGAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)
Plasmid#195049Purposeintegration cassette for ku70 deletion and expression of NAT gene under pPGI for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpPGIAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-GGvT
Plasmid#87362PurposeExpresses GGvT, a tandem homodimer green fluorescent proteinDepositorInsertGGvT
TagsHis-TagExpressionBacterialAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-11
Plasmid#134803PurposeIPTG inducible expression of S-TAGA-Evo1 qtRNADepositorInsertWildtype S tRNA (decodes TCG)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT892
Plasmid#68933PurposeInt9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertInt9
ExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbPpep-CHis
Plasmid#48243PurposeFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domainDepositorInsertClbP-pep
Tags6-HisExpressionBacterialMutationFirst 375 amino acids of the peptidase, which con…PromoterT7Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
NAT2
Plasmid#25182PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINO_F2
Plasmid#171926PurposeBacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_F2
TagsHis6ExpressionBacterial, Insect, and Mamm…Available SinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-beta1,4 GT-1
Plasmid#11872DepositorInsertbeta 1,4 galactosyltransferase (B4GALT1 Human)
ExpressionBacterialAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -