30,577 results

1. pET21a-LeuRS-His

   Plasmid

   #124113

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Leucyl-tRNA ligase (leuS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGNW2

   Plasmid

   #122086

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19_CDC45_dTAG

   Plasmid

   #232733

   Purpose

   Generates CDC45 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   CDC45 (CDC45 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX459_MCM2_sgRNA

   Plasmid

   #232730

   Purpose

   Generates MCM2 dTAG C ternimal knock-in cell lines

   Depositor

   Junjie Chen

   Insert

   MCM2 (MCM2 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NO

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459_GINS4_gRNA

   Plasmid

   #232734

   Purpose

   Generates GINS4 dTAG C ternimal knock-in cell lines

   Depositor

   Junjie Chen

   Insert

   GINS4 (GINS4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NO

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX459_CDC45_sgRNA

   Plasmid

   #232732

   Purpose

   Generates CDC45 dTAG C ternimal knock-in cell lines

   Depositor

   Junjie Chen

   Insert

   CDC45 (CDC45 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NO

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19_GINS4_dTAG

   Plasmid

   #232735

   Purpose

   Generates GINS4 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   GINS4 (GINS4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19_MCM2_dTAG

   Plasmid

   #232731

   Purpose

   Generates MCM2 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   MCM2 (MCM2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a-TrpRS-His

   Plasmid

   #124120

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Tryptophanyl-tRNA ligase (trpS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET_SZ1-RGG-RGG

    Plasmid

    #124942

    Purpose

    Expression of SZ1-RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mOrange2-pBAD

    Plasmid

    #54531

    Purpose

    Localization: Protein Expression Vector , Excitation: 549, Emission: 565

    Depositor

    Nathan Shaner, Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mOrange2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZ8-T_dCas9

    Plasmid

    #74062

    Purpose

    pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    dcas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-CHC(1-1074)-His6

    Plasmid

    #100748

    Purpose

    Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CLTC (CLTC Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Residues 1-1074

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-32a(+)-NRG1B1

    Plasmid

    #120287

    Purpose

    Produce human recombinant NRG1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    NRG1-beta 1
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRH2521

    Plasmid

    #84380

    Purpose

    Expression of sgRNA from a imyc promoter (Pimyc)

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    sgRNA cloning site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRH2502

    Plasmid

    #84379

    Purpose

    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    dcas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate at position 10 changed to Alanine, Hist…

    Promoter

    uv15tetO

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX4T moesin 1-322

    Plasmid

    #11638

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    moesin (MSN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino-terminal half of moesin.

    Promoter

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

19. S96 ELP

    Plasmid

    #68393

    Purpose

    Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    S96 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBA2219

    Plasmid

    #38152

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

    Insert

    DsbC (dsbC E. coli)
    

    Use

    Tags

    N-terminal leader peptide (cloning artifact)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge