30,738 results

1. 3xFLAG-CUL1-pCMV7.1

   Plasmid

   #155019

   Purpose

   N-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 1 (CUL1 Human)
   

   Tags

   3xFLAG

   Expression

   Bacterial

   Promoter

   pCMV7.1

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTPH412

   Plasmid

   #185724

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-gp41-8N
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   ecTadA(8e) R26G

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAWP78-PVCpnf_pvc13-antiHistoneNb

   Plasmid

   #198293

   Purpose

   Produces PVCs retargeted with A9 anti-histone nanobody

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-antiHistoneNb
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFPC1-muscle Amphiphysin 2

   Plasmid

   #22213

   Depositor

   Pietro De Camilli

   Article

   Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

   Insert

   Amphiphysin II (BIN1 Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pR6K-Donor(PmcCAST)_KanR

   Plasmid

   #224928

   Purpose

   Transposition assay

   Depositor

   Leifu Chang

   Article

   Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

   Insert

   LE_cargo_RE
   

   Expression

   Bacterial

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-28a-YTH domain of YTHDC2

   Plasmid

   #228779

   Purpose

   Protein expression plasmid for YTH domain of YTHDC2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDC2 (YTHDC2 Human)
   

   Tags

   6*His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-TetOminiCMV-OSK

   Plasmid

   #136552

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQTEV-UFD1L

   Plasmid

   #31560

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   ubiquitin fusion degradation 1-like (UFD1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FLAG-Synaptojanin 1-170

   Plasmid

   #22292

   Depositor

   Pietro De Camilli

   Article

   Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

   Insert

   Synaptojanin 1_170 (SYNJ1 Human)
   

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   K334R compared to Genbank ID NM_003895

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pFP.R268

    Plasmid

    #138180

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJUMP47-2A(sfGFP)

    Plasmid

    #126991

    Purpose

    Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTR47m4-GFP

    Plasmid

    #102436

    Purpose

    Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

    Depositor

    Gregory Stephanopoulos

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    Formaldehyde repressor protein (frmR)
    Superfolder GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Reb_206 aka PAS629

    Plasmid

    #71234

    Purpose

    Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    mNeonGreen to second copy RebB, soluble mCherry a…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTS-hFluc

    Plasmid

    #177716

    Purpose

    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Tags

    Mitochondrial Targeting Sequence MTS SS

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE60-EFTu-His

    Plasmid

    #124127

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor Tu (tufB Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Miguel Angel Moreno-Mateos, Julián Cerón

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMega-MaPylRS

    Plasmid

    #200225

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    proK-lacO and tacI

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbB2a-GFP

    Plasmid

    #35343

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET16b-His-ArgRS

    Plasmid

    #124104

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Argin+I4 ligase (argS Escherichia coli)
    

    Tags

    10x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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