30,416 results

1. pET His6 MBP TEV LIC cloning vector (2M-T)

   Plasmid

   #29708

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRESCA-syntide

   Plasmid

   #132415

   Purpose

   FRET-based sensor for CaMKII activity

   Depositor

   Margaret Stratton

   Article

   Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

   Insert

   FRESCA-syntide
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1(nsp8-nsp7)(nsp12)

   Plasmid

   #165451

   Purpose

   Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

   Depositor

   Marc Delarue

   Article

   Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

   Inserts

   nsp12
   nsp8
   nsp7
   

   Use

   Tags

   14Histidine-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule-tfmF A65V S158A

   Plasmid

   #85484

   Purpose

   Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32V F108W Q109M I159M with 65Val and 158Ala

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 12.4kbVillin-ΔATG

   Plasmid

   #19358

   Depositor

   Deborah Gumucio

   Article

   Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

   Insert

   12.4kb Villin promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No ATG - transgenes cloned downstream of the vill…

   Promoter

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOSIP-TT (TetR, P21)

   Plasmid

   #45989

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-HA-BI-AR

   Plasmid

   #171234

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

    Plasmid

    #80840

    Purpose

    Modified helper phage

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene II
    M13 gene X
    M13 gene V
    M13 gene VII
    M13 gene IX
    M13 gene VIII
    M13 gene I
    M13 gene XI
    M13 gene IV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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