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Bacterial Expression

30,577 results

Showing: 2501 - 2520 of 30577 results

  1. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Promoter
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  4. pRECMYC-Ebu1

    Plasmid
    #240713
    Purpose
    pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Insert
    Ebu1 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240713-plasmid-map-sequence-id-482476

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  6. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 constitutive promoter and native promoter
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  9. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Martin Reijns, Andrew Jackson
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  10. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid
    #172506
    Purpose
    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    DisCas7-11 bacterial expression vector with golden gate site for guides
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172506-plasmid-map-sequence-id-344928

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  11. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJEx (Jungle Express)
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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  12. pET-alpha-synuclein-BiFC

    Plasmid
    #87855
    Purpose
    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    alpha-synuclein-VC155
    alpha-synuclein-VN154
    Use
    Tags
    C-terminal fragment of mVenus BiFC protein and N-…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87855-plasmid-map-sequence-id-175017

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  18. pTOX5

    Plasmid
    #127450
    Purpose
    Encodes MqsR toxin and amilCP chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    mqsR toxin
    amilCP chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    pRha and tac
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127450-plasmid-map-sequence-id-249680

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Showing: 2501 - 2520 of 30577 results