30,577 results

1. pGEX-4T1 human PP2A Aα full length

   Plasmid

   #132634

   Purpose

   expresses human PP2A Aα full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

   Insert

   PP2A Aα (PPP2R1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSP1E1

   Plasmid

   #20062

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1E1 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1; contains repeat of hel…

   Promoter

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-His-IF1

   Plasmid

   #124123

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 1 (infA Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TDP-43_CTD_M337V

   Plasmid

   #98674

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M337V

   Promoter

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6p1-GST-Ubiquitin

   Plasmid

   #223236

   Purpose

   Bacterial expression construct for GST-Tagged Ubiquitin production and purification

   Depositor

   Weixing Zhao

   Insert

   Ubiquitin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE80l ACA

   Plasmid

   #196471

   Purpose

   Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   ACA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHEN-CoV2-nanobody-C7

   Plasmid

   #194591

   Purpose

   Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   C7 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-45b-mCherry-NPM1

   Plasmid

   #194546

   Purpose

   Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   NPM1 (Npm1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26b_Nb.b201

    Plasmid

    #131404

    Purpose

    E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody b201
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMS31: pTwinStrep-SUMO-PmcTnsAB

    Plasmid

    #168164

    Purpose

    PmcTnsAB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsAB
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-SMAD4

    Plasmid

    #16491

    Depositor

    Bert Vogelstein

    Article

    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pAN1201

    Plasmid

    #74695

    Purpose

    Circuit backbone plasmid (LacZa insert)

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET15-MHL

    Plasmid

    #26092

    Purpose

    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. CD11b promoter in pGEM3zf(-)

    Plasmid

    #26168

    Depositor

    Daniel Tenen

    Article

    Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

    Insert

    CD11b promoter (ITGAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE30-His-IF3

    Plasmid

    #124125

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 3 (infC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a IHF

    Plasmid

    #26641

    Depositor

    Phoebe Rice

    Insert

    IHF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only