29,712 results

1. pET251-pUC 12xMS2V6 Loxp KANr Loxp

   Plasmid

   #104392

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCT5-bac1.8

   Plasmid

   #119871

   Purpose

   Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

   Depositor

   Claudia Schmidt-dannert

   Article

   Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

   Inserts

   CymR'
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   SNP in 592 bp

   Promoter

   PserA promoter and Pveg promoter with CuO sequence

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET-mSA-EGFP

   Plasmid

   #39862

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin fused to EGFP
   

   Use

   Tags

   6xHis, EGFP, and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule-tfmF A65V S158A

   Plasmid

   #85484

   Purpose

   Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32V F108W Q109M I159M with 65Val and 158Ala

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETa_ mEos4b

   Plasmid

   #51073

   Purpose

   Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

   Depositor

   Loren Looger

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   mEos4b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACRISPR-KM

   Plasmid

   #203587

   Purpose

   A sgRNA expression plasmid for genome editing in Burkholderia

   Depositor

   Leonilde Moreira

   Article

   Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AF1521-c002

   Plasmid

   #173109

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD/His-B-iRFP

   Plasmid

   #31855

   Purpose

   a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

   Depositor

   Vladislav Verkhusha

   Article

   Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

   Insert

   iRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

   Promoter

   araBAD

   Available since

   Sept. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7C-HSP90AA1

   Plasmid

   #177658

   Purpose

   Recombinant protein synthesis of human HSP90AA1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSP90AA1 (HSP90AA1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-Padron2

    Plasmid

    #172355

    Purpose

    Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

    Depositor

    Stefan Jakobs

    Article

    Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

    Insert

    Padron2
    

    Use

    Tags

    6xHisTag (N terminal on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLI1_pet28a

    Plasmid

    #131641

    Purpose

    Bacterial expression of ETS gene FLI1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLPT20

    Plasmid

    #85460

    Purpose

    Repressilator with integrated reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    PLlac, PLtet, PR, and PRNAI

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWJB9

    Plasmid

    #126650

    Purpose

    Expresses AdrA from a modified pLAFR3

    Depositor

    Ute Romling

    Article

    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

    Insert

    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Use

    Tags

    no tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINK-OTUB1 (full-length, aa 1-271)

    Plasmid

    #61420

    Purpose

    Expresses human OTUB1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. ELK1_pet28a

    Plasmid

    #131647

    Purpose

    Bacterial expression of ETS gene ELK1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDIV297

    Plasmid

    #204820

    Purpose

    AMA1 plasmid with amdS selection marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Insert

    amdS
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations (nt177T>C, nt879C>T)

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-His6-Ubc9

    Plasmid

    #133909

    Purpose

    Bacterial expression of His6-Ubc9

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSETB PKA RIalpha

    Plasmid

    #14922

    Depositor

    Susan Taylor

    Article

    Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

    Insert

    PKA R1alpha regulatory subunit (PRKAR1A Bovine)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge