30,260 results

1. pCDB179-TfCut2-S121P-D174S-D204P

   Plasmid

   #218695

   Purpose

   Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TfCut2
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   S121P-D174S-D204P

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T-1 3xFlag-MAPKAPK2

   Plasmid

   #82720

   Purpose

   Expresses human MAPKAPK2

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MAPKAPK2 (MAPKAPK2 Human)
   

   Use

   Tags

   3xFLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pEGFPC1-EPSIN 1

   Plasmid

   #22228

   Depositor

   Pietro De Camilli

   Article

   Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

   Insert

   Epsin 1 (Epn1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   please see depositor comment below

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLADE_ONLY_A

   Plasmid

   #168051

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Insert

   Blue light-inducible AraC dimers in Escherichia coli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101**

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T1-14-3-3 zeta GST

   Plasmid

   #13278

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

   Insert

   14-3-3 zeta (YWHAZ Bovine)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pMAL-c5x_UnaG

   Plasmid

   #163125

   Purpose

   A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   UnaG
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

   Plasmid

   #190790

   Purpose

   [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Inserts

   Cas9
   mazF-LVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   LVA

   Expression

   Bacterial

   Mutation

   silent mutations of BpiI-sites in original Cas9 g…

   Promoter

   PnrsB and Ptrc-[E*](104)

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFPN1-human Dynamin 1aa K44A

   Plasmid

   #22197

   Depositor

   Pietro De Camilli

   Article

   Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

   Insert

   human Dynamin 1aa K44A (DNM1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   K44A

   Promoter

   Available since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBB535

    Plasmid

    #27392

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ (dnaJ, dnaK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSFDuet-1_VNp-mNeongreen

    Plasmid

    #182387

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB1H1-Zif268

    Plasmid

    #12612

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 in pB1H1 (Egr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-KIRIN1

    Plasmid

    #107414

    Purpose

    Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET23 P53DBD

    Plasmid

    #174503

    Purpose

    express P53 DNA binding domain (94-312) in E.coli without tag

    Depositor

    Stewart Loh

    Article

    Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

    Insert

    P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKL001

    Plasmid

    #98917

    Purpose

    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    CaPR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. L4440_BioBrick-SCR-sgRNA-B

    Plasmid

    #177812

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    Scrambled sgRNA targeting sequences
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CYP2C19

    Plasmid

    #69606

    Purpose

    For protein expression of CYP2C19

    Depositor

    Joyce Goldstein

    Insert

    CYP2C19 (CYP2C19 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deletion of AA1-8 and mutation I331V

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b_Q69L

    Plasmid

    #186272

    Purpose

    Expression vector of His-RanQ69L in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Use

    Tags

    His-tagged at N terminal

    Expression

    Bacterial

    Mutation

    Changed Q69 into L

    Promoter

    T7 promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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