30,577 results

1. pMSP1E1

   Plasmid

   #20062

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1E1 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1; contains repeat of hel…

   Promoter

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15-MHL

   Plasmid

   #26092

   Purpose

   SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Sept. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. CD11b promoter in pGEM3zf(-)

   Plasmid

   #26168

   Depositor

   Daniel Tenen

   Article

   Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

   Insert

   CD11b promoter (ITGAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-SMAD4

   Plasmid

   #16491

   Depositor

   Bert Vogelstein

   Article

   Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

   Insert

   Smad4 (SMAD4 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

5. mCarmine in pRSETB

   Plasmid

   #109484

   Purpose

   bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

   Depositor

   Oliver Griesbeck

   Article

   Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

   Insert

   mCarmine
   

   Use

   Tags

   N-terminal histidine tag in vector

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-45b-mCherry-NPM1

   Plasmid

   #194546

   Purpose

   Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   NPM1 (Npm1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-His-IF3

   Plasmid

   #124125

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 3 (infC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TDP-43_CTD_M337V

   Plasmid

   #98674

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M337V

   Promoter

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid

    #189752

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PARG-c002

    Plasmid

    #173107

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARG (PARG Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S448-T976

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6p1-GST-Ubiquitin

    Plasmid

    #223236

    Purpose

    Bacterial expression construct for GST-Tagged Ubiquitin production and purification

    Depositor

    Weixing Zhao

    Insert

    Ubiquitin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE80l ACA

    Plasmid

    #196471

    Purpose

    Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

    Depositor

    Fei Sun

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

    Insert

    ACA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHEN-CoV2-nanobody-C7

    Plasmid

    #194591

    Purpose

    Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    C7 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P2-MDM2 (17-125)

    Plasmid

    #62063

    Purpose

    Expression of human MDM2 (17-125) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 17-125

    Promoter

    tac

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH6HTC_HSPA9-Halo

    Plasmid

    #175332

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HSPA9 (HSPA9 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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