32,967 results
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Plasmid#82479Purposereporter of GAPDH expressionDepositorAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only
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pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
TagsHis tag and LAA degradation tagExpressionBacterialAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLC-246
Plasmid#73190PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-tse2
ExpressionBacterialPromoterPrhaBAvailable SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 (XSB363)
Plasmid#78505PurposeConstruct for expressing Grb2 in E. ColiDepositorInsertGrb2 (GRB2 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
Tags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCM18
Plasmid#78565Purpose60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmidDepositorTypeEmpty backboneTagsluxCDABEExpressionBacterialAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAUC40
Plasmid#71298PurposeSuicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GATE-16
Plasmid#73518PurposeFor recombinant expression of human GATE-16/GABARAPL2 in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLicC-MBP-APETx2
Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
TagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialPromoterT7Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp-ecfp
Plasmid#65034PurposeTpR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-Rbck1
Plasmid#63135PurposeExpression of human RBCK1/HOIL-1LDepositorInsertHOIL-1L (RBCK1 Human, Synthetic)
TagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_41
Plasmid#59964PurposeHigh full length T7 RNAP* expressionDepositorInsertT7* RNAP
ExpressionBacterialMutationN/APromoterPJ23105Available SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HisYFP-Sp100
Plasmid#53141PurposeE.coli expression of HisYFP tagged human Sp100 fragmentDepositorInsertSp100A fragment (SP100 Human)
TagsHis-YFPExpressionBacterialMutationfragment (amino acids 71-480); deletion of L75PromoterT7Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
ExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CH-GECO2.1
Plasmid#52099PurposemCherry-based Calcium indicatorsDepositorInsertCH-GECO2.1
Tags6 His tagExpressionBacterialPromoterpBAD promotorAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMal-E6LLO
Plasmid#51281PurposeExpresses a fibronectin fusion with listeriolysin targeting EGFR in bacteriaDepositorInsertE6LLO
ExpressionBacterialAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQR
Plasmid#48099PurposeCumate-inducible expression of mCherry-tagged fusion proteinDepositorTypeEmpty backboneTags3xFLAG and mCherryExpressionBacterialPromoterPQ5Available SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRBBm34
Plasmid#48114PurposeExpression of the gfp gene under control of the native xylose inducible promoter of Bacillus megateriumDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-luxCDABE-term
UseSynthetic BiologyExpressionBacterialPromoterPesaRAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-SNX3
Plasmid#31573DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only