30,577 results

1. pMS31: pTwinStrep-SUMO-PmcTnsAB

   Plasmid

   #168164

   Purpose

   PmcTnsAB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsAB
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a IHF

   Plasmid

   #26641

   Depositor

   Phoebe Rice

   Insert

   IHF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pAN1201

   Plasmid

   #74695

   Purpose

   Circuit backbone plasmid (LacZa insert)

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBP-T7RNAP

   Plasmid

   #74096

   Purpose

   T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE30-His-RF3

   Plasmid

   #124131

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 3 (prfC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG79-attL1-pegRNA-attL2

   Plasmid

   #213050

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST39

   Plasmid

   #64009

   Purpose

   original polycistronic cloning vector for plasmid suite (generation I)

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET His6 NusA TEV LIC cloning vector (1N)

   Plasmid

   #29657

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, NusA, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWP78-PVCpnf_pvc13-E01DARPin

   Plasmid

   #198287

   Purpose

   Produces PVCs retargeted with anti-EGFR DARPin E01

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-E01DARPin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-slpGFP

    Plasmid

    #27171

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET9d-hnRNP-A1

    Plasmid

    #23026

    Depositor

    Douglas Black

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEB1-mVenus NB

    Plasmid

    #103986

    Purpose

    Plasmid encoding mVenus NB

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus NB
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ2072.2_CRISPRlator

    Plasmid

    #140674

    Purpose

    CRISPRlator

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    CRISPRlator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQTEV-LRPAP1

    Plasmid

    #31327

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    low density lipoprotein receptor-related protein associated (LRPAP1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSH124-ssr

    Plasmid

    #198025

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Ap/Cb resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ApR promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Use

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV, p10

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH2B_mCherry_IRES_neo3

    Plasmid

    #21044

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEM10686

    Plasmid

    #213995

    Purpose

    PrpsM driven expression of mScarlet-I

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE60-EFTs-His

    Plasmid

    #124128

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor Ts (tsf Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHis-BRD4 BD1

    Plasmid

    #196544

    Purpose

    Expression of BRD4 Bromodomain (BD1) in E.coli

    Depositor

    Byung Il Lee

    Article

    Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

    Insert

    BRD4 BD1 (BRD4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge