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33,441 results

Showing: 2601 - 2620 of 33441 results

  1. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85496-plasmid-map-sequence-id-169865

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  2. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    85498-plasmid-map-sequence-id-169862

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  5. pNR220

    Plasmid
    #79541
    Purpose
    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.
    Depositor
    Timothy Lu
    Publication
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    A118
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79541-plasmid-map-sequence-id-148753

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  6. pSLC-246

    Plasmid
    #73190
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Publication
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-tse2
    Expression
    Bacterial
    Promoter
    PrhaB
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73190-plasmid-map-sequence-id-151367

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  7. pMBP-SpyCatcher

    Plasmid
    #79053
    Purpose
    Expresses maltose binding protein (MBP) SpyCatcher fusion
    Depositor
    James Bowie
    Publication
    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi
    Insert
    MBP-SpyCatcher
    Tags
    6xHis and Maltose binding protein
    Expression
    Bacterial
    Mutation
    Derived from pDEST14-SpyCatcher (Plasmid #35044)…
    Promoter
    T7
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    79053-plasmid-map-sequence-id-147896

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  16. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Publication
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2601 - 2620 of 33441 results