30,602 results

1. pRsetB-pHRed

   Plasmid

   #31472

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLASP_mScarlet_CLASP_RGS2membrane

   Plasmid

   #133086

   Purpose

   Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   mScarlet-CLASP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NNMT

   Plasmid

   #40734

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NNMT (NNMT Human)
   

   Use

   Tags

   His Thrombin

   Expression

   Bacterial

   Mutation

   K100A:E101A:E103A

   Promoter

   Available Since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. TGT-His

   Plasmid

   #138201

   Purpose

   Expresses E. Coli TGT with a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Insert

   E Coli tRNA Guanine Transglycosylase-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-TS2126 RNA Ligase

   Plasmid

   #119941

   Purpose

   Express TS2126 RNA ligase in E. coli

   Depositor

   Takashi Ito

   Article

   Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCgCas9_recT

   Plasmid

   #153337

   Purpose

   Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTrcHIS-7D12Gln13TAG

   Plasmid

   #125264

   Purpose

   Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   Gln13TAG 7D12
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET_sfCherry(1-10)_32aalinker_sfCherry(11)

   Plasmid

   #83030

   Purpose

   Bacterial expression vector for split sfCherry screening

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry(1-10)_32aalinker_sfCherry(11)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

    Plasmid

    #47580

    Purpose

    Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Inserts

    MKK4-EE (MAP2K4 Human)
    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks the first 36 residues of the RefSeq protein…

    Promoter

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pBTK1010 (pSL25)

    Plasmid

    #191005

    Purpose

    Expresses blue chromoprotein on a broad host range RP4 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    blue chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNP2157

    Plasmid

    #222732

    Purpose

    pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Helper (WT)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-HA-AR-DBD

    Plasmid

    #171238

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE60-RRF-His

    Plasmid

    #124132

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Ribosome recycling factor (frr Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSET.HaloTag.v7.AviTag

    Plasmid

    #195486

    Purpose

    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    HaloTag.v7.AviTag
    

    Use

    Tags

    6xHIS and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACM4

    Plasmid

    #49797

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Use

    Tags

    S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. V96 ELP

    Plasmid

    #68392

    Purpose

    Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V96 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFVK5

    Plasmid

    #167522

    Purpose

    Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    VanRAM-mKelly2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PVanCC

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSK9065

    Plasmid

    #84345

    Purpose

    tightly controlled expression of mRFP fusion proteins in S. aureus

    Depositor

    Neville Firth

    Article

    Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

    Type

    Empty backbone

    Use

    Tags

    mRFPmars

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl/tet

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TOPO mKate2

    Plasmid

    #68441

    Purpose

    TOPO construct expressing mKate2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mKate2
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge