30,743 results

1. pNDGG054

   Plasmid

   #231323

   Purpose

   BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVP143

   Plasmid

   #222080

   Purpose

   Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

   Insert

   dCas9-PmCDA1, sfGFP, sacB and AeBlue
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCN-EF2132tet

   Plasmid

   #107191

   Purpose

   Expresses EF2132 recombinase for performing recombineering in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   EF2132
   tetR
   

   Expression

   Bacterial

   Promoter

   p23

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD.Sigma32.I54N

   Plasmid

   #59982

   Purpose

   Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

   Depositor

   Jeffery Kelly

   Article

   Zhang et al ACS Chem Biol. 2014 Jul 22.

   Insert

   Sigma32 I54N
   

   Expression

   Bacterial

   Mutation

   I54N

   Available Since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE60-RRF-His

   Plasmid

   #124132

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Ribosome recycling factor (frr Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLICSFAST-LDh-LY-P2A

   Plasmid

   #214895

   Purpose

   Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-LDh-LY-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbS5k-RFP

   Plasmid

   #35285

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hchk2 (kin-)-PUNI10

   Plasmid

   #41908

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   CHK2 (CHEK2 Human)
   

   Expression

   Bacterial

   Mutation

   kinase dead (D347A)

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET17_K560_GFP_His

   Plasmid

   #15219

   Depositor

   Ron Vale

   Article

   Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

   Insert

   Human neuronal kinesin heavy chain (KIF5B Human)
   

   Tags

   GFP and His

   Expression

   Bacterial

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJCSUP35(1-253)

    Plasmid

    #1089

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 1 - 253 of SUP35

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pBP-T7RNAP

    Plasmid

    #74096

    Purpose

    T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKCcas9dO

    Plasmid

    #62552

    Purpose

    High efficiency Streptomyces genome editing by CRISPR/Cas9 system

    Depositor

    Weihong Jiang

    Article

    Huang et al Acta Biochim Biophys Sin (Shanghai). 201

    Inserts

    Scocas9
    sgRNA ()
    act-orf4 homology arm
    

    Use

    CRISPR; Bacterial genome editing

    Expression

    Bacterial

    Promoter

    j23119 and ptipA

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-NTOM20-mVenus-Zdk2

    Plasmid

    #81011

    Purpose

    Expression of Zdk2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk2
    

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFPC1-human APPL1

    Plasmid

    #22198

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIB184 (EM)

    Plasmid

    #90194

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Tags

    None

    Expression

    Bacterial

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDC216

    Plasmid

    #115791

    Purpose

    Expresses rat Syntaxin 1

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Syntaxin1 (Stx1a Rat)
    

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    C145S S193C C271S C272S

    Promoter

    T7

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS6

    Plasmid

    #55752

    Purpose

    Produces T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    T7 RNA Polymerase
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLS.492

    Plasmid

    #184957

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE30-hPolB

    Plasmid

    #70761

    Purpose

    bacterial expression of human DNA Polymerase beta (POLB)

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    POLB (POLB Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    R333L

    Promoter

    T5/lac operon

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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