30,260 results

1. GST-Ub4

   Plasmid

   #171421

   Purpose

   For the purifcation of tetra linear ubiquitin

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   Ubiquitin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPH6

   Plasmid

   #40315

   Depositor

   Shaun Lott

   Article

   Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

   Insert

   Peptide:N-glycosidase F
   

   Use

   Tags

   6xHis and OmpA3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB46 (CK2beta)

   Plasmid

   #27085

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL004

   Plasmid

   #215336

   Purpose

   amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)

   Depositor

   Gene-Wei Li

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hyper-pSpank

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b_RanWT

   Plasmid

   #186270

   Purpose

   Expression vector of His-RanQ69L in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Use

   Tags

   His tagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYAL_HLA-A2_MART1

   Plasmid

   #171177

   Purpose

   Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

   Depositor

   Michael Birnbaum

   Article

   Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

   Insert

   MART1_HLA-A2*01 single-chain trimer fusion
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-pA-Mnase

   Plasmid

   #124883

   Purpose

   To transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.

   Depositor

   Keji Zhao

   Article

   Ku et al Genome Res. 2021 Apr 14. pii: gr.260893.

   Insert

   protein A-MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6_MBP_FMRP_P451S

   Plasmid

   #198693

   Purpose

   Expression of human FMRP protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FMR1 (FMR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed proline 451 to serine

   Promoter

   T7

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTO_CHMP4B_LAP

   Plasmid

   #101850

   Purpose

   Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

   Depositor

   Daniel Gerlich

   Article

   Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

   Insert

   Charged Multivesicular Body Protein 4B (CHMP4B Human)
   

   Use

   Gateway cloning, flp-frt recombination, tet-induc…

   Tags

   Localization and Affinity Purification (LAP) tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-huMb3Cas12a

    Plasmid

    #174677

    Purpose

    Expresses 6xHis-MBP tagged Mb3Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb3Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xHis-MBP-huMb2Cas12a

    Plasmid

    #174676

    Purpose

    Expresses 6xHis-MBP tagged Mb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BK Dunlop

    Plasmid

    #25466

    Depositor

    Peter Howley

    Insert

    human papovavirus BK Dunlop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pACYC-FLAG-dN6-His

    Plasmid

    #22143

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    Flag-clpXdeltaNLinkedHexamer-His6
    

    Use

    Tags

    Flag, His6, and L20 Linkers

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK CA RhoA

    Plasmid

    #112894

    Purpose

    promoter-inducible expression of constitutively-active RhoA

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    CA RhoA (RHOA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHYRS52

    Plasmid

    #31122

    Depositor

    Hideo Iwai

    Article

    Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

    Insert

    Ulp1 (ULP1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. HHM-iATPSnFR1.0

    Plasmid

    #102546

    Purpose

    expresses ATP sensor in bacteria

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVoPo-01

    Plasmid

    #187836

    Purpose

    Backbone for transcriptional reporter system in Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-2(KW)2-eGFP

    Plasmid

    #112736

    Purpose

    Express 2(KW)2-eGFP in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-eGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM2s2AsG

    Plasmid

    #137924

    Purpose

    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSK+KanaRpsL

    Plasmid

    #20871

    Depositor

    Jiyue Zhu

    Article

    Wang et al Mol Biotechnol. 2009 May;42(1):110-6. do

    Insert

    KanamycinResistance-RpsL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge