33,441 results
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Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pCRISPomyces-FnCpf1
Plasmid#129554PurposeContains codon-optimized FnCpf1 and gRNA for streptomycesDepositorInsertFnCpf1
ExpressionBacterialPromoterrpsL(XC)-BbsIAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-gpASNase1
Plasmid#121526PurposeExpresses gpASNase1 in mammalian cells.DepositorInsertgpASNase1
UseRetroviralTagsFLAGExpressionBacterial and MammalianPromoterCMVAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPEPZ-Plac
Plasmid#122635PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pATLIC
Plasmid#110287PurposeA backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)DepositorInsertyfaL
ExpressionBacterialPromoteraraBAD promoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30 PTBP1 isoform1
Plasmid#108591PurposeRecombinant His tagged protein productionDepositorPublicationAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE135
Plasmid#118489Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
ExpressionBacterialAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLind
Plasmid#113109PurposeVector encoding the RFP gene under blue light-inducible promoter BindF1.DepositorInsertRFP
UseCell-freeExpressionBacterialPromoterBindF1Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mRuby2
Plasmid#111250PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)DepositorInsertmRuby2
ExpressionBacterialMutationmRuby2 is codon optimized for expression in P. fl…PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-Full-Length-MePCE
Plasmid#113539PurposeProduces an N-terminal GST-tagged full length MePCE protein.DepositorAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSF-T20S
Plasmid#110805Purposeexpresses Thermoplasma acidophilium 20S proteasome in E. ColiDepositorInsertsPsmB
PsmA
Tagstev-HisExpressionBacterialMutationN2D and Q2EPromoterT7 and T7 promoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
TagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mTurquoise2
Plasmid#108235PurposeBroad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPc promoter - mTurquoise2
ExpressionBacterialMutationcodon optimized for experession in P. fluorescensAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ
Plasmid#109380PurposeRhamnose inducible LacZ expression. Used to test burden response in E. coliDepositorInsertpRHAM-LacZ
ExpressionBacterialAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL004
Plasmid#98920PurposeConstitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.DepositorInsertGCaMP6f tethered to mRuby
TagsHis and mRuby3ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
Tags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-hcGAS(157-522)
Plasmid#108676PurposeFor expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522DepositorInsertcGAS amino acid 157-522 (CGAS Human)
TagsGSTExpressionBacterialMutationcontains amino acid 157-522PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBb(B5)1k-GFPuv
Plasmid#106393PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only