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pBT29-splitD

Plasmid

#122599

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHJL1

Plasmid

#199376

Purpose

Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression

Depositor

Hang Lu

Insert

GCaMP6s::SL2::mScarlet-I

Expression

Bacterial

Mutation

no mutations

Available Since

April 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMRE140

Plasmid

#118492

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG_OriM

Plasmid

#110097

Purpose

Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJCC_076 AsCas12a R1226A

Plasmid

#171669

Purpose

For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a R1226A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginine 1226 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFC331

Plasmid

#87843

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
argB

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMT85_SpdCas9_pmcDA1

Plasmid

#204614

Purpose

CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_neo3

Plasmid

#21044

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pJUMP27ts-1A(sfGFP)

Plasmid

#126975

Purpose

Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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5WCI (MYST1)

Plasmid

#101269

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

MYST1 (KAT8 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pUC18 3xFLAG COP1

Plasmid

#237334

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

COP1 (COP1 Human)

Expression

Bacterial

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 dUBA (1-389)

Plasmid

#210426

Purpose

Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL dUBA (1-389) (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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IRES-creERT2-FNF

Plasmid

#188230

Purpose

EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette

Depositor

Mario Capecchi

Insert

IRES-creERT2-FNF

Use

Cre/Lox and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Hela kinase-dead MLCK-GFP

Plasmid

#46317

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase-dead (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

E1626K

Promoter

CMV

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only
pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--PrecA-YFP

Plasmid

#228097

Purpose

recA promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTW386c

Plasmid

#237321

Purpose

expresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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F-IRF7

Plasmid

#169777

Purpose

Express IRF7 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)

Tags

Flag tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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