30,260 results

1. pAW016-2

   Plasmid

   #85613

   Purpose

   Cas9-tracrRNA integration vector for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   cas9-tracrRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExp-MsyB

   Plasmid

   #112570

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8His and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC- AVITAG-CTH10STII- -GG

   Plasmid

   #215805

   Purpose

   Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET-6xHis-mCherry(-6)

   Plasmid

   #205045

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   mCherry(-6)
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRN3P-NLS-EGFP-Btgh-3NLS

   Plasmid

   #194469

   Purpose

   Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

   Depositor

   Mathieu Boulard

   Article

   Formichetti et al Dev Cell 10 Jan 2024

   Insert

   BtGH84
   

   Use

   For in vitro transcription from t3 promoter after…

   Tags

   SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-mNeonGreen-IRESpuro2

   Plasmid

   #183745

   Purpose

   Expresses H2B-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScaf-5544.1

   Plasmid

   #111405

   Purpose

   pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

   Depositor

   Shawn Douglas

   Article

   Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

   Insert

   pScaf-GH'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pλPR cI-Elysis

   Plasmid

   #176891

   Purpose

   Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

   Depositor

   James Inglese

   Article

   Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

   Insert

   Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2/HB

   Plasmid

   #26557

   Depositor

   Perry Hackett

   Insert

   pT2/HB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rb-379-928-pGex2TK

    Plasmid

    #21749

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928 of wt Rb …

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSP1E3D1_D73C

    Plasmid

    #39328

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E3D1_D73C (APOA1 synthetic gene)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cys mutant of MSP1E3D1

    Promoter

    T7

    Available since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

    Plasmid

    #135456

    Purpose

    Bacterial expression of uTEV2Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, T30A/S153N mut…

    Promoter

    tac promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ADA

    Plasmid

    #39020

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ADA (ADA Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAF-HdrB3

    Plasmid

    #184908

    Purpose

    carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cas9-sgRNA-homology arms
    

    Use

    CRISPR; Broad-host-range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEN6-Ubc5 Sc

    Plasmid

    #83234

    Purpose

    pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Inserts

    Ubiqutin (UBC Human)
    Ubc 5 (UBC5 Budding Yeast)
    Uba 1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB076i3

    Plasmid

    #79198

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-C2-mKate

    Plasmid

    #208763

    Purpose

    To express C2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-mKelly1

    Plasmid

    #119782

    Purpose

    Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly1
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. APneg-VAMP1d

    Plasmid

    #168010

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET251-pUC 12xMS2V6 Loxp KANr Loxp

    Plasmid

    #104392

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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