30,744 results

1. pRSFDuet-1_mNeongreen-Erythropoietin

   Plasmid

   #182422

   Purpose

   Bacterial expression of mNeongreen-Erythropoietin fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen-eythropoietin (EPO Human)
   

   Tags

   mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

   Plasmid

   #182423

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-eythropoietin (EPO Human)
   

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXT128_TwinStrep-SUMO-ShCas12k

   Plasmid

   #135524

   Purpose

   Protein expression construct for ShCast12k

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShCas12k
   

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPP6534

   Plasmid

   #128735

   Purpose

   Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB208

   Plasmid

   #164123

   Purpose

   Ptac driving PP7 repeats

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   YFP-PP7
   

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBluescript-CTG-47

   Plasmid

   #99150

   Purpose

   47xCAG repeats in pBluescript vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   47xCTG
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-mKelly2

   Plasmid

   #119783

   Purpose

   Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

   Depositor

   Stephen Mayo

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

   Insert

   mKelly2
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG2_hph

   Plasmid

   #184915

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEasyG2_mic

   Plasmid

   #184916

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJUMP49-2E(sfGFP)

    Plasmid

    #126995

    Purpose

    Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX4T1 SHP1 WT

    Plasmid

    #8578

    Depositor

    Ben Neel

    Insert

    SHP1 (PTPN6 Human)
    

    Expression

    Bacterial

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJUMP29-1D(sfGFP)

    Plasmid

    #126963

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJUMP29-1C(sfGFP)

    Plasmid

    #126962

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJUMP29-1B(sfGFP)

    Plasmid

    #126961

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-DAXX-D/E-His

    Plasmid

    #179619

    Purpose

    Express DAXX D/E region in bacteria

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Tags

    GST, His, and TEV

    Expression

    Bacterial

    Mutation

    amino acids 414-505 of DAXX

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30-R5-GFP

    Plasmid

    #17838

    Depositor

    Jay Keasling

    Article

    Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

    Insert

    pET30-GFP
    

    Use

    Synthetic Biology

    Tags

    His, R5, and S-tag

    Expression

    Bacterial

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. bNS2B47NS3

    Plasmid

    #155320

    Purpose

    co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJUMP29-1E(sfGFP)

    Plasmid

    #126982

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCheY Tc delta C

    Plasmid

    #13520

    Depositor

    James McKerrow

    Article

    Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

    Insert

    cruzain
    

    Tags

    CheY

    Expression

    Bacterial

    Mutation

    Truncated variant: stop codon after Gly212

    Available Since

    March 11, 2009

    Availability

    Academic Institutions and Nonprofits only