30,260 results

1. pETDuet-1 Hepatitis C Virus NS3/NS4A

   Plasmid

   #61696

   Purpose

   inducible dual expression of NS3 protease along with its cofactor NS4A

   Depositor

   Michael Johnson

   Article

   Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

   Inserts

   NS4A
   NS3
   

   Use

   Tags

   his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCoofy1-psHaloTag1a

   Plasmid

   #232940

   Purpose

   Photoswichable HaloTag: psHaloTag1a variant

   Depositor

   Claire Deo

   Article

   Walterspiel et al bioRxiv 2024.12.18.629107

   Insert

   photoswitchable HaloTag 1a variant
   

   Use

   Tags

   His6x-HRV3C

   Expression

   Bacterial

   Mutation

   E143W, A258W with linkers L1 (FAG) and L2 (P)

   Promoter

   T7

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-tdTEVDB_C-15A/G4C

   Plasmid

   #177205

   Purpose

   Permuted td intron with evolved initiation sequence for the expression of circular mRNA

   Depositor

   Stephen Fried

   Article

   Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

   Insert

   Permuted GFP with evolved initiation sequence for circular mRNA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Permuted sfGFP after residue 52 & Changed Cyt…

   Promoter

   araBAD

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15-UbE2D2

   Plasmid

   #15783

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D2 (UBE2D2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pKSI-1

   Plasmid

   #51725

   Purpose

   cloning backbone

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human LFA-1 alpha

   Plasmid

   #8630

   Depositor

   Timothy Springer

   Article

   Larson et al J Cell Biol 1989 Feb;108(2):703-12.

   Insert

   LFA1 alpha (ITGAL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

7. Pet22b-mEGFP

   Plasmid

   #166439

   Purpose

   Bacterial expression of 6x His tagged mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. BBa_J72114-BBa_J72104

   Plasmid

   #40783

   Depositor

   Christopher Anderson

   Article

   Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

   Insert

   RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ
   

   Use

   Luciferase and Synthetic Biology; Phagemid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTECH-MmPylRS(IPYE)

   Plasmid

   #104073

   Purpose

   Expression plasmid encoding MmPylRS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmPylRS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTR1

    Plasmid

    #22468

    Depositor

    Gary Pielak

    Article

    Olteanu et al Biochem Biophys Res Commun. 2003 Dec 19.

    Insert

    cytochrome c (CYCS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas1+2

    Plasmid

    #72676

    Purpose

    Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

    Depositor

    Udi Qimron

    Article

    Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

    Insert

    Cas1, Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNH-NanoLuc

    Plasmid

    #173075

    Purpose

    Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC-fFuCas9-HTBNLS-hph

    Plasmid

    #121092

    Purpose

    Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

    Depositor

    Xiao-Jun Ji

    Article

    Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

    Insert

    Cas9 and hygromycin resist gene
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gpdA;trpC

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYTK002

    Plasmid

    #65109

    Purpose

    Encodes ConLS as a Type 1 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWJ66

    Plasmid

    #46570

    Purpose

    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9-w
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC574

    Plasmid

    #127975

    Purpose

    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    hygB (hygromycin resistance marker)
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX4T1-Opt WWP1

    Plasmid

    #179327

    Purpose

    E. coli optimized expression of GST-fusion of human WWP1

    Depositor

    Arthur Haas

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPS22 (pET23a-Cas10/Csm/csm2H6N)

    Plasmid

    #70038

    Purpose

    Bacterial expression of the S. epidermidis Cas10·Csm complex

    Depositor

    Luciano Marraffini

    Article

    Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

    Insert

    Cas10/Csm complex
    

    Use

    Tags

    6xHIS on N terminus of Csm2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. SUMO_MT3

    Plasmid

    #200303

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1delZn2delBRCT

    Plasmid

    #173939

    Purpose

    Bacterial expression of PARP1 lacking Zn2 and BRCT domains

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2delBRCT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 and 374-520; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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