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cisRAJ31-GFP-LAA

Plasmid

#69928

Purpose

Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-GFP-LAA

Use

Synthetic Biology

Expression

Bacterial

Promoter

PlLacO1

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Tags

His and V5

Expression

Bacterial and Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCON1-ProC.gfp

Plasmid

#112517

Purpose

ProC driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProC gfpmut3 TT

Expression

Bacterial

Promoter

ProC

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T moesin 307-577

Plasmid

#11639

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

moesin (MSN Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of moesin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pCYP29

Plasmid

#212862

Purpose

Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDV02

Plasmid

#212156

Purpose

Plasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Article

Tan et al ACS Synth Biol. 2023 Jul 21;12(7):2041-2

Insert

secondary alcohol dehydrogenase

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pUS252

Plasmid

#127674

Purpose

Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones

Depositor

Nicholas Coleman, Mark Somerville

Insert

Free Use GFP (fuGFP)

Expression

Bacterial

Promoter

lac

Available Since

June 24, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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PLEXIN A1

Plasmid

#58237

Purpose

Riboprobe for in vitro transcription of Plexin A1

Depositor

Christiana Ruhrberg

Article

Bouvree et al Circ Res. 2012 Aug 3;111(4):437-45. doi:

Insert

Plexin A1 (Plxna1 Mouse)

Expression

Bacterial

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-hXRCC1

Plasmid

#70759

Purpose

bacterial expression of human XRCC1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

XRCC1 (XRCC1 Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-Rac1

Plasmid

#12200

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

Starting methionine (M1) removed; F78S compared t…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pBbA8k-RFP

Plasmid

#35273

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET3d-E2-25K-C170S

Plasmid

#18892

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin conjugating enzyme E2-25K (UBE2K Most likely H. sapiens, Human)

Expression

Bacterial

Mutation

Cysteine 170 is replaced with a Serine residue.

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pGEM4Z-Fluc

Plasmid

#183474

Purpose

This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

Depositor

Chantal Pichon

Article

Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

Insert

Firefly luciferase

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP1187

Plasmid

#119256

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHBJT034

Plasmid

#225180

Purpose

Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT023

Plasmid

#225174

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pJC20_cam1-T6C

Plasmid

#182427

Purpose

Bacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1 with the T6C residue replacement to allow labelling

Depositor

Dan Mulvihill

Article

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

cam1 (cam1 Fission Yeast)

Expression

Bacterial

Mutation

T6C substitution

Promoter

T7

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-loop

Plasmid

#132695

Purpose

pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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