29,744 results

1. NP-11-64-1

   Plasmid

   #234359

   Purpose

   Expressing rubisco and prk in Δrpi

   Depositor

   David Savage

   Article

   Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

   Insert

   R.rubrum rubisco
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-MBP-Cdc42-Q61L

   Plasmid

   #214288

   Purpose

   Express constitutively active Cdc42 fused with MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Constitutively active Cdc42 (CDC42 Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Cdc42-Q61L

   Promoter

   Tac

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACS125

   Plasmid

   #204593

   Purpose

   GST-lamin A C522A (389-646)

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   human lamin A C522A (389-646)
   

   Use

   Tags

   GST - lamin A C522A (389-646)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pACS45

   Plasmid

   #204581

   Purpose

   GST-lamin A (389-646)

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   lamin A (389-646)
   

   Use

   Tags

   GST - lamin A (389-646)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMB3-B-TelR

   Plasmid

   #204992

   Purpose

   Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET17b-yebST

   Plasmid

   #175804

   Purpose

   Complementation of yebST knockout in E. coli K-12

   Depositor

   Damian Ekiert, Ian Henderson, Gira Bhabha

   Article

   Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

   Insert

   yebST
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   silent mutations to introduce restriction sites

   Promoter

   T7

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAGH42

   Plasmid

   #107249

   Purpose

   Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

   Depositor

   Erin O'Shea

   Article

   Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

   Insert

   vipp1-gfp
   

   Use

   Suicide vector for destined for double homologous…

   Tags

   mGFPmut3

   Expression

   Bacterial

   Mutation

   Promoter

   native promoter of sll0617

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQ-Elo-HB-C-A(1-630)

   Plasmid

   #171086

   Purpose

   Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   Elongin B (ELOB Human)
   Elongin C (ELOC Human)
   Elongin A (ELOA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deleted N-term 26aa and C-term 142aa for mutant e…

   Promoter

   T5 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNCS mHoneydew

    Plasmid

    #91760

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Honeydew
    

    Use

    Tags

    no tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Plasmid

    #159580

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psgRNAv1-empty

    Plasmid

    #171611

    Purpose

    sgRNA_v1 plasmid for AsCas12f1 in bacteria

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1_sgRNA_1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T-1 3xFlag-CREB

    Plasmid

    #82721

    Purpose

    Expresses human CREB

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    CREB (CREB1 Human)
    

    Use

    Tags

    3XFLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SENP7 (catalytic domain)

    Plasmid

    #16360

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP7 catalytic domain (SENP7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 640 to 984 catalytic domain of human S…

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pSW036

    Plasmid

    #140034

    Purpose

    KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Yellow fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cpt

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-2xMreBN-mCherry-His

    Plasmid

    #133626

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    2xMreB-mCherry-His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET His6 ProteinG TEV LIC cloning vector (1P)

    Plasmid

    #29660

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, Protein G, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-5X1 Flag WDR5

    Plasmid

    #15553

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Wdr5 (Wdr5 Mouse)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only