30,753 results

1. pGEX SHP-2(NC)-SH2

   Plasmid

   #46499

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(NC) (PTPN11 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-222

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1-Zn2

   Plasmid

   #174805

   Purpose

   Bacterial expression of isolated PARP1 Zn2 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn2 (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1-Zn3

   Plasmid

   #174806

   Purpose

   Bacterial expression of isolated PARP1 Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn3 (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDmDERusk

   Plasmid

   #213702

   Purpose

   Vector for red-light-induced bacterial gene expression

   Depositor

   Andreas Moeglich

   Article

   Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNP1047

   Plasmid

   #222726

   Purpose

   pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSKBAD2

   Plasmid

   #15335

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein
   

   Tags

   GB1

   Expression

   Bacterial

   Available Since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP4_2A4D

   Plasmid

   #234196

   Purpose

   Heterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2A4D (UBE2V1 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET30b_OmpAT7Sav

   Plasmid

   #138589

   Purpose

   T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

   Insert

   T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
   

   Use

   Synthetic Biology

   Tags

   OmpA export signal and T7 tag

   Expression

   Bacterial

   Promoter

   PT7

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. His6_MBP_FXR1P

   Plasmid

   #198694

   Purpose

   Expression of human FXR1 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR1P (FXR1 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pChuA

    Plasmid

    #42539

    Depositor

    Alan Jasanoff

    Article

    Lelyveld et al J Am Chem Soc. 2011 Feb 2;133(4):649-51.

    Insert

    ChuA (chuA E. coli O157:H7)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_RGG-GFP-RGG

    Plasmid

    #124948

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    EGFP and His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB1A3_GFP_dT23

    Plasmid

    #89663

    Purpose

    expression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT23
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nme2Cas9_pMCSG7

    Plasmid

    #119925

    Purpose

    Nme2Cas9 bacterial expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Tags

    NLS, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEThT-CAHS3

    Plasmid

    #90028

    Purpose

    To express His-tagged CAHS3 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS3
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-HsSENP2

    Plasmid

    #71466

    Purpose

    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pSB1A3_GFP_dT34

    Plasmid

    #89669

    Purpose

    expression of doubleTAL staple protein dT34 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT34
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET19b-rDAD2 cPGFP(G164)

    Plasmid

    #165994

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(G164)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. OmpF

    Plasmid

    #213135

    Purpose

    Expresses outer membrane protein F

    Depositor

    Sebastian Hiller

    Insert

    OmpF (23-362) (ompF E.coli (K12))
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCP20T-mPlum

    Plasmid

    #78474

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    mPlum
    

    Expression

    Bacterial

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-AviTag-DogTag-MBP

    Plasmid

    #171773

    Purpose

    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogTag-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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