33,906 results
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Plasmid#202631PurposeEnables dual growth and fluorescence based enrichment of RamR biosensorsDepositorInsertSh ble
UseSynthetic BiologyExpressionBacterialPromoterLambda promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRExpressionBacterialAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis-BRD4 BD1
Plasmid#196544PurposeExpression of BRD4 Bromodomain (BD1) in E.coliDepositorAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb
Plasmid#198292PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobodyDepositorInsertPVCpnf_pvc13-antiMouseMHCIINb
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE2D3
Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
TagsnoneExpressionBacterialPromoterT7Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN-sacB
Plasmid#196249PurposePlasmid containing the sacB geneDepositorInsertattR2-sacB-attL3
ExpressionBacterialPromotersacB promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBAD-HisD-miniGFP2
Plasmid#188897PurposeExpress 6xHis-Tag miniGFP2 in E.coliDepositorInsertminiGFP2
Tags6xHisExpressionBacterialPromoteraraBADAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
TagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBolux
Plasmid#188109PurposeEncodes a promoter-less Bacteroides-optimized lux cassetteDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcoFus
Plasmid#187833Purpose1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpressionDepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-hfYFP
Plasmid#186522PurposeHyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertHyperfolder YFP
Tags6xHisExpressionBacterialMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
ExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBA620
Plasmid#186240PurposeLbuCas13a Negative Control (RFP targeting)DepositorInsertpTet-LbuCas13a + crRNA negative control
ExpressionBacterialAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFC0619
Plasmid#186690PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargoDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
ExpressionBacterialPromoterPLacAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHPSE-P6
Plasmid#185907PurposeEngineered Human Heparanase mutant for e.coli expressionDepositorTagsHis-tagExpressionBacterialPromoterT7Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDae029 (eMutaT7[TadA-8e])
Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
ExpressionBacterialAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mut1-RS
Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
ExpressionBacterialAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
ExpressionBacterialAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only