30,753 results

1. pGEX-KG-WASP-GBD

   Plasmid

   #30113

   Depositor

   David Sacks

   Article

   Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-

   Insert

   WASP-GBD (WAS, WAS Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains the Cdc42-interacting domain of WASP (aa…

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-hisMBP-AsCpf1-EC

   Plasmid

   #79007

   Purpose

   Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   AsCpf1
   

   Tags

   6xHis-MBP-NLS and NLS-HA

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF-HdrB3

   Plasmid

   #184908

   Purpose

   carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cas9-sgRNA-homology arms
   

   Use

   CRISPR; Broad-host-range

   Expression

   Bacterial

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG8Ha-EWS-FLI1_Type2

   Plasmid

   #188042

   Purpose

   Expresses His8-EWS-FLI1(TypeII) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   EWS-FLI1 (EWSR1 Human)
   

   Tags

   His8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PG1424PGA

   Plasmid

   #74652

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PG1424PGA
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pET11a-SUMO2

   Plasmid

   #53142

   Purpose

   E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   SUMO2 (SUMO3 Human)
   

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-92)

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAP05

   Plasmid

   #186261

   Purpose

   sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   sfGFP
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Expression

   Bacterial

   Promoter

   BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-BirA-eCPX-AP

   Plasmid

   #121907

   Purpose

   Expresses BirA and eCPX-AP

   Depositor

   Per Svenningsen

   Article

   Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

   Inserts

   BirA (birA Synthetic)
   Enhanced Circularly Permuted Outer Membrane Protein X
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 and pBAD promoter

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTW199b2

    Plasmid

    #136928

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-PhoCl1-6His

    Plasmid

    #164033

    Purpose

    Expressed PhoCl1 in a pET expression vector

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl1
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b-CPDSalI

    Plasmid

    #38251

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIRCNpu

    Plasmid

    #74226

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Npu DnaE C-intein and Npu DnaE N-intein

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSW003-Pc-mNeonGreen

    Plasmid

    #205018

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-2.3k-fabp2

    Plasmid

    #84927

    Purpose

    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector

    Depositor

    John Rawls

    Insert

    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    

    Tags

    NO

    Expression

    Bacterial

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG031 (upx)

    Plasmid

    #157909

    Purpose

    Expresses the upx defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    upx (putative PD-(D/E)xK family phosphodiesterase)
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pScI_dCas9-CDA-UL

    Plasmid

    #108551

    Purpose

    Bacterial Target-AID UGI-LVA vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1-UGI-LVA
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPP089

    Plasmid

    #158796

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Expression

    Bacterial

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMTBX-2

    Plasmid

    #26073

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-LIC+

    Plasmid

    #62312

    Purpose

    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and thrombin protease cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only