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31,059 results

Showing: 3881 - 3900 of 31059 results

  3. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

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  5. EHMT2

    Plasmid
    #25503
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT2 (EHMT2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pHN1002

    Plasmid
    #178831
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    His8, 3C
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178831-plasmid-map-sequence-id-351933

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  11. Ydf NanoLuc

    Plasmid
    #225656
    Purpose
    Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NanoLuciferase
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Promoter
    PydfJ115
    Available Since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225656-plasmid-map-sequence-id-455061

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  12. pJL NarL YdfI Flag

    Plasmid
    #225650
    Purpose
    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NarL-YdfI (narL Synthetic)
    Use
    Cell free expression
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    NarL with native DNA-binding domain replaced with…
    Promoter
    T7
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    225650-plasmid-map-sequence-id-454890

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  14. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163127-plasmid-map-sequence-id-331491

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  19. pASPIre2

    Plasmid
    #154843
    Purpose
    Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Expression
    Bacterial
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154843-plasmid-map-sequence-id-307634

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Showing: 3881 - 3900 of 31059 results