30,753 results

1. enz.099-PKA

   Plasmid

   #154048

   Purpose

   Expresses PKA in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
   

   Tags

   His, MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCPP3373

   Plasmid

   #128718

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopQ1-1
   

   Expression

   Bacterial

   Mutation

   Synonymous point mutation in the wobble position…

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mem-mCherry-pTRIP

   Plasmid

   #163521

   Purpose

   Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   membrane targeted mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET30(a)-AtNUDX23

   Plasmid

   #175021

   Purpose

   Purification of AtNUDX23 enzyme

   Depositor

   Jeppe Vinther

   Article

   Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

   Insert

   AtNUDX23 (NUDX23 Mustard Weed)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQE-60 roGFP2-His

   Plasmid

   #65046

   Purpose

   Bacterial expression of His-tagged roGFP2

   Depositor

   Tobias Dick

   Insert

   roGFP2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   see sequence

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLRD11

   Plasmid

   #208263

   Purpose

   Production of sclareol in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   abCAS
   ssSS
   

   Expression

   Bacterial

   Mutation

   abCAS(D537A)

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXD68Kan2-AphA

   Plasmid

   #191248

   Purpose

   RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBADTrfp

   Plasmid

   #99382

   Purpose

   This promoter system provided the highest RFP expression upon induction

   Depositor

   Nathan Hillson

   Insert

   RFP
   

   Expression

   Bacterial

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJE53

   Plasmid

   #194153

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available Since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pBS mCD4

    Plasmid

    #14613

    Depositor

    Dan Littman

    Article

    Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

    Insert

    CD4 (Cd4 Mouse)
    

    Expression

    Bacterial

    Available Since

    June 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET51b-His-TEV-SNAP-tag_fast

    Plasmid

    #167271

    Purpose

    Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB061i

    Plasmid

    #79226

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-HA4; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    Ppro1; PlacIq

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPBO.ACT001

    Plasmid

    #182711

    Purpose

    aTc inducible dCasRx

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    2x-FLAG

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFUSE-rIgG-Fc2-ICAM1

    Plasmid

    #156462

    Purpose

    Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

    Depositor

    Dirk Mielenz

    Article

    Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

    Insert

    Intercellular Adhesion Molecule 1 (Icam1 Mouse)
    

    Tags

    ICAM1-rFc

    Expression

    Bacterial and Mammalian

    Mutation

    endogenous signal peptide replaced with IL2 signa…

    Promoter

    hEF1-HTLC

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT413

    Plasmid

    #8606

    Depositor

    Robert Hartley

    Article

    Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

    Insert

    barnase, barstar
    

    Tags

    phoA signal sequence to barnase

    Expression

    Bacterial

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

    Plasmid

    #30505

    Depositor

    Ichiro Matsumura

    Article

    Murin et al Appl Environ Microbiol. 2011 Oct 21.

    Insert

    beta-glucuronidase (gusA Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDDGFP-A

    Plasmid

    #40286

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    ddGFP-A
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a-VHH-myc

    Plasmid

    #109422

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge