29,747 results

1. pBad-PhoCl2f

   Plasmid

   #164036

   Purpose

   Improved PhoCl variant with faster dissociation rate

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2f
   

   Use

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBad promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTHSSe_50

   Plasmid

   #109248

   Purpose

   incW BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY002 (pFnCpf1_min)

   Plasmid

   #69975

   Purpose

   Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 locus without Cas genes nd artificial promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-iPASnFR-L

   Plasmid

   #225238

   Purpose

   Bacterial expression of iPASnFR-L

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. nifF in pET21b

   Plasmid

   #124520

   Purpose

   Azotobacter vinelandii flavodoxin II of Nitrogenase

   Depositor

   Douglas Rees

   Article

   Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

   Insert

   Flavodoxin II (nifF Azotobacter vinelandii)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / lac

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Grp1-PH pGEX2TK

   Plasmid

   #71379

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 264-391

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pMJ825

   Plasmid

   #39315

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A)

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRedCm

   Plasmid

   #176225

   Purpose

   LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB and PL

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA24N_6xHis-TEV-rpsA

   Plasmid

   #83585

   Purpose

   Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

   Depositor

   Nils Walter

   Article

   Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

   Insert

   rpsA (rpsA Escherichia coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   p-T5 Lac

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AK5

    Plasmid

    #39136

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK5 (AK5 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pSLC-237

    Plasmid

    #73191

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    ParaB-relE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ParaB

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAF

    Plasmid

    #104152

    Purpose

    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

    Depositor

    Robert Craigie

    Article

    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

    Insert

    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    

    Use

    Tags

    N-terminal His tag with thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease

    Plasmid

    #228637

    Purpose

    Bacterial expression of the Coxsackievirus 16 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Coxsackievirus 16 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pNMTMA_MBP-Cav1-His6

    Plasmid

    #223700

    Purpose

    To produce standard caveospheres (without the Z-domain), Parton Lab clone LDB

    Depositor

    Rob Parton

    Article

    T. D. et al bioRxiv

    Insert

    MBP-Cav1-His6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Nano-lantern(cAMP-1.6)/pRSETB

    Plasmid

    #53591

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pVRa41_491

    Plasmid

    #49702

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF41_491
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDae029 (eMutaT7[TadA-8e])

    Plasmid

    #187620

    Purpose

    Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    TadA-8e-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSETA-PAGFP

    Plasmid

    #11911

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Insert

    pRSETA-PAGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The PAGFP has four mutations (L64F/T65S/V163A/T20…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. hsp70: mKate2-DN Kif5a

    Plasmid

    #105972

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Kif5a (kif5aa Zebrafish)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    truncation of aa416-1033

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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