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Plasmid#141135Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionBacterialMutationD3 deletion, I32F in Zdk2PromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSG360
Plasmid#115601Purposesurface-displayed Ag3; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogTag-MBP
Plasmid#171773PurposeExpresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogTag-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
SS9_RNA
Plasmid#71656PurposeSS9-targeting gRNA for co-transformation with pSS9DepositorInsertSS9 gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-11_enzymatic-domain
Plasmid#183382PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-11 (AA100-373) (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT86
Plasmid#108965PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tagDepositorTypeEmpty backboneUseTagsOmpA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF RBP-FL P53
Plasmid#174042PurposeExpress Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)DepositorInsertP53 gene (TP53 Human)
UseTagsThermoanaerobacter Tencongenesis ribose binding p…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREDusk-AmpR-MCS
Plasmid#188974PurposeFor red light-repressed bacterial expression of gene of interest (AmpR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAJM.661
Plasmid#108532PurposeTtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.DepositorInsertTtgRAM + PTtg-YFP
UseTagsExpressionBacterialMutationTtgR: R130H A144T A148VPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutH
Plasmid#86609PurposeE. coli MutH expression plasmidDepositorInsertE. coli MutH
UseTagshis-tag and ald-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
Plasmid#117656PurposeBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.DepositorInsertssfCherry2(1-10)
sfCherry2(11)-SpyCatcher002
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2R2
Plasmid#15806DepositorInsertUbiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LY-MT-P2A
Plasmid#214897PurposeExpresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sitesDepositorInsertSPLICSFAST-LY-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-140
Plasmid#118524PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutS
Plasmid#86605PurposeE. coli MutS expression plasmidDepositorInsertE. coli MutS (D835R,R840E)
UseTagshis-tag and ald-tagExpressionBacterialMutationD835R, R840EPromoterT7Available sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCC1-4k-BBclone2
Plasmid#137070PurposeE. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1DepositorInsertsBB0323
P13
DipA
Lmp1
UseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PERK 5: PERK.K618R.pGEX4T-1
Plasmid#21818DepositorInsertPERK (537-1114) K618R (Eif2ak3 Mouse)
UseTagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114. Kinase …PromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcNB1
Plasmid#89241PurposeResurfaced Cell-penetrating GFP nanobodyDepositorInsertpcNB1 (GFP)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWUKI 1+2
Plasmid#79879PurposeEncodes Cas1+2 and a CRISPR arrayDepositorInsertsCas1
Cas2
CRISPR Array
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only