31,793 results
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Plasmid#213864PurposeBinary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.DepositorInsertchloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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GST-Rab8a-wt
Plasmid#86078PurposeA bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.DepositorAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-StayGold
Plasmid#211360PurposeBacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertStayGold
Tags10xHis-tagExpressionBacterialPromoterT7Available SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-iRFP670
Plasmid#45453DepositorInsertiRFP670
ExpressionBacterialMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoteraraBADAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPP381
Plasmid#158798PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-2
Tags6xHisExpressionBacterialMutationRuvC-inactivating mutation D394AAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialPromoterT7Available SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA
Plasmid#176743PurposeExpresses Connexin-43 fused with BioID2 at the C-terminus in astrocytesDepositorInsertCx43-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC3-FGFR1
Plasmid#222611PurposeExpression of MAC3-tagged FGFR1DepositorAvailable SinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDD108
Plasmid#119884PurposeIntegration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
ExpressionBacterialAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
p346_DG
Plasmid#234676PurposeGolden gate domesticated pET28b with His10-TEVDepositorTypeEmpty backboneTagsHis10-TEVExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKCcas9dO
Plasmid#62552PurposeHigh efficiency Streptomyces genome editing by CRISPR/Cas9 systemDepositorInsertsScocas9
sgRNA ()
act-orf4 homology arm
UseCRISPR; Bacterial genome editingExpressionBacterialPromoterj23119 and ptipAAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDM2 (17-125)
Plasmid#62063PurposeExpression of human MDM2 (17-125) in e. coliDepositorInsertMDM2 (MDM2 Human)
TagsGSTExpressionBacterialMutationcontains residues 17-125PromotertacAvailable SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMBP-AaCas12b
Plasmid#113433PurposeExpresses Expresses 10xHis-MBP fused to AaCas12bDepositorInsertAaCas12b
TagsMBPExpressionBacterialPromoterT7Available SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL10-mRuby-iATPSnFR1.0 bb118
Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
Tagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Profiline
Plasmid#89925PurposeBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actinDepositorAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT
Plasmid#46987PurposeInducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
TagsGST and PreScission cleavage siteExpressionBacterialMutationcontains amino acids 190–530 of accession NP_0562…PromotertacAvailable SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRelA*
Plasmid#175590PurposeTet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
TagsFused with a flexible GS linker to mVenusExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pProEx-IDE-wt
Plasmid#99014PurposeExpress human Insulin Degrading Enzyme (IDE) in E. coli.DepositorAvailable SinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Spike-403-505
Plasmid#165095Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
ExpressionBacterialAvailable SinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits