31,793 results
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Plasmid#129503PurposeExpresses AausGFP constitutively in E. coli (most strains)DepositorInsertAausGFP
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)
Plasmid#136627PurposePlasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-LAMA-R59
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-YedK
Plasmid#86884PurposeBacterial expression of E. coli SRAP ortholog YedKDepositorAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NLyn-Venus-Zdk1
Plasmid#81042Purposemammalian expression of plasma-membrane anchored Zdk1DepositorInsertZdk1
Tags6x His tag, FLAG, NLyn, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-monomer-hAIM2
Plasmid#51544PurposeExpress DsRed monomer-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDB180
Plasmid#80677Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged proteinDepositorTypeEmpty backboneTagsOsmY-10His-Smt3ExpressionBacterialPromoterT7Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK 14-3-3 sigma GST
Plasmid#11944DepositorAvailable SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHIS-GB1-BP1
Plasmid#133427PurposeHuman BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.DepositorInsertSREBF1 (SREBF1 Human)
ExpressionBacterialMutationSREBP1a amino acids 17-42 fused to GB1 solubilit…Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJ826
Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7Available SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAKlux2.1
Plasmid#14081DepositorInsertBacterial luciferase
ExpressionBacterialAvailable SinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBAD_sfGFP3TCG4TAG
Plasmid#174518PurposeRecoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.DepositorInsertsfGFP
Tags6 x HisExpressionBacterialMutationRecoded. TCG and TAG codons were introduced in po…PromoteraraBADAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG1C
Plasmid#71740PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11d-RAN
Plasmid#108919PurposeExpresses human RANDepositorInsertRAN (RAN Human)
ExpressionBacterialAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1 ZIKV E
Plasmid#145341PurposeCell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFPDepositorInsertZIKV Envelope
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ISA431: aTc-MP6
Plasmid#189935PurposeMutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanRDepositorInsertdanQ926, dam, seqA, emrR, ugi and cda1
ExpressionBacterialPromoterpTetAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-TEV-Nrf2
Plasmid#62455PurposeHalo-His6 tagged Nrf2 for E.coli expressionDepositorAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only