30,753 results

1. pHis APEX2-eIF4A1

   Plasmid

   #129645

   Purpose

   Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   eukaryotic translation initiation factor 4A1 (EIF4A1 Human)
   

   Tags

   6xHis and APEX2

   Expression

   Bacterial

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-TEX264_wt-His6

   Plasmid

   #220214

   Purpose

   Bacterial expression of wild type TEX264, C-terminal His6 tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE135

   Plasmid

   #118489

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-80L MBP-SspB Nano

   Plasmid

   #60409

   Purpose

   E. coli expression of SspB Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   SspB nano
   

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   Y11K and A15E

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Cis1-SH2

   Plasmid

   #46417

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Cis1 (SOCS1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 90-214

   Promoter

   Tac

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSG323

   Plasmid

   #115594

   Purpose

   surface-displayed Null

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMan operon

   Plasmid

   #124888

   Purpose

   Expresses proteins encoding Man operon in E. faecalis

   Depositor

   Kazuhisa Sekimizu

   Article

   Matsumoto et al Commun Biol. 2019 May 2;2:157. doi: 10.1

   Insert

   manX1, manX2, manY, manZ, manO, EF0025
   

   Expression

   Bacterial

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3R112C/T180CBM3

   Plasmid

   #85090

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The R112C/T180C variant of PCNA3 fused to P450 BM3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

   Promoter

   T7 promoter

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCP20T-morange

   Plasmid

   #78468

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   mOrange
   

   Expression

   Bacterial

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniTn7(Gm)PA1/04/03-eyfp-a

    Plasmid

    #111620

    Purpose

    eYFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-eyfp
    

    Expression

    Bacterial

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-Streptavidin-K121R

    Plasmid

    #89880

    Purpose

    Streptavidin that has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K121R-Glutamate_Tag
    

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K121R

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFP.E222

    Plasmid

    #138248

    Purpose

    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET41b+_Ufd1-His

    Plasmid

    #117107

    Purpose

    Production of recombinant Ufd1-His in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Ufd1 (UFD1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. rbcLS-pMAL-2px

    Plasmid

    #41621

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    rbcL/rbcS
    

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_GNAI1_G-alpha

    Plasmid

    #110012

    Purpose

    Protein expression and purification of GNAI1_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI1_G-alpha (GNAI1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCool-mRbx1-His-hCul1-CTP

    Plasmid

    #29519

    Depositor

    Ning Zheng

    Insert

    Rbx1 CUL1 (CUL1, Rbx1 Human, Mouse)
    

    Tags

    GST, His (before thrombin), and Thrombin Cut Site…

    Expression

    Bacterial

    Available Since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCoofy9

    Plasmid

    #122004

    Purpose

    E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    StrepII

    Expression

    Bacterial

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ELAC1

    Plasmid

    #39097

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ELAC1 (ELAC1 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pBAD_GFP11_T7LysH17A

    Plasmid

    #59591

    Purpose

    Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

    Depositor

    Richard Kingston

    Article

    Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

    Type

    Empty backbone

    Tags

    6xHis-tag and GFP11

    Expression

    Bacterial

    Promoter

    pARA

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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