29,754 results

1. pBbB8k-GFP

   Plasmid

   #35363

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BAD

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINB-OTUB1*

   Plasmid

   #65441

   Purpose

   Expresses activated OTUB1 in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   UbcH5b (UBE2D2 Human)
   otubain-1 (OTUB1 )
   

   Use

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Active site cysteine mutated to alanine

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. PA-Cdc42

   Plasmid

   #75263

   Purpose

   Light-activated Cdc42

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   Cell Division Cycle 42 (CDC42 Human)
   

   Use

   Tags

   6xHIs, LOV2, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   F56W

   Promoter

   CMV

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLumi-LgBiT

   Plasmid

   #188319

   Purpose

   Expression of LgBiT via sigma 70 promoter in E. coli

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   LgBiT
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sigma 70 promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SS9_RNA

   Plasmid

   #71656

   Purpose

   SS9-targeting gRNA for co-transformation with pSS9

   Depositor

   Ryan Gill

   Article

   Bassalo et al ACS Synth Biol. 2016 Apr 22.

   Insert

   SS9 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b-MBPx-MBPx-SpyTag003

   Plasmid

   #216291

   Purpose

   Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MBPx-MBPx-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

   Plasmid

   #117656

   Purpose

   Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Inserts

   sfCherry2(1-10)
   sfCherry2(11)-SpyCatcher002
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Mouse ZP3 promoter (JD#445)

   Plasmid

   #14634

   Depositor

   Jurrien Dean

   Article

   Rankin et al Development. 1998 Jul . 125(13):2415-24.

   Insert

   ZP3 promoter (Zp3 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZ854

   Plasmid

   #208116

   Purpose

   mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mNeonGreen-HPRT1-Neo
   

   Use

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZ852

    Plasmid

    #208114

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-HygTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-HygTK
    

    Use

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30-T7-His-sfGFP

    Plasmid

    #180755

    Purpose

    pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid

    #169191

    Purpose

    To express nsp10-nsp16 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

    Insert

    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM-30-GST-PfAgo_F

    Plasmid

    #146265

    Purpose

    Bacterial Expression of PfAgo

    Depositor

    Elisa Izaurralde

    Insert

    PfAgo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent T1704C and one non silent mutation A90…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFW2100

    Plasmid

    #224212

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T3-RhoA-Q63L

    Plasmid

    #12961

    Depositor

    Gary Bokoch

    Insert

    RhoA constitutively active (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Q63L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a.Tat(48-57)-EGFP

    Plasmid

    #165040

    Purpose

    Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    EGFP
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 3xFLAG-CUL3-pCMV7.1

    Plasmid

    #155021

    Purpose

    N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 3 (CUL3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-Ran(Q69L)

    Plasmid

    #42048

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Ran (RAN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q69L

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-21a(+)/MBP-His-PINK1

    Plasmid

    #38005

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only