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pJB153
Plasmid#92328PurposeExpress mEos3.2 tagged ZapA in E coliDepositorInsertZapA
UseTagsmEos3.2ExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.D2-NP
Plasmid#134366PurposeNanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.DepositorInsertD2-NP (DRD2 Human)
UseTagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-132
Plasmid#118550PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-p53 (1-90) (72R)
Plasmid#62081PurposeExpression of human p53 (TAD residues 1-90) (72R) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationContains TAD/PP residues 1-90, 72RPromotertacAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2T
Plasmid#15808DepositorInsertUbiquitin conjugating enzyme UbE2T (UBE2T Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSK+KanaRpsL
Plasmid#20871DepositorInsertKanamycinResistance-RpsL
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBAT5
Plasmid#112581PurposeUsed for expression of protein in E. coli as non-fusions without initiation ATGDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY001 (pFnCpf1_full)
Plasmid#69973PurposeExpresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.DepositorInsertFnCpf1 locus
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
2NVR
Plasmid#51340PurposeCatalytic domain of human histone deacetylase HDAC7DepositorInsertHDAC7
UseTagsHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Tn5
Plasmid#127917PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.DepositorInsertTn5
UseTagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7Available sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MITF-M (wt)
Plasmid#31151DepositorInsertMITF-M (MITF Human)
UseTagsFLAGExpressionBacterial and MammalianMutationWTPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCM18
Plasmid#78565Purpose60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmidDepositorTypeEmpty backboneUseTagsluxCDABEExpressionBacterialMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRG2
Plasmid#149474PurposeBroad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertRhaS-RhaR-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
P48
Plasmid#212155PurposePlasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.DepositorInsertsuperfolder green fluorescent protein, secondary alcohol dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37
Plasmid#196657PurposePlasmid for overexpression of native initiator tRNA (A37)DepositorInserttRNAfMet-A37 expression cassette
UseTagsExpressionBacterialMutationPromotermetY native promoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b_Q69L
Plasmid#186272PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis-tagged at N terminalExpressionBacterialMutationChanged Q69 into LPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE161
Plasmid#118509Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only
