30,513 results

1. pET264-pUC 24xMS2V6 Loxp KANr Loxp

   Plasmid

   #104393

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

   Plasmid

   #35152

   Depositor

   Jay Keasling, Taek Soon Lee

   Article

   Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

   Insert

   AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   R364S in Mevalonate Kinase

   Promoter

   LacUV5, pTrc

   Available Since

   May 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRH2521

   Plasmid

   #84380

   Purpose

   Expression of sgRNA from a imyc promoter (Pimyc)

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   sgRNA cloning site
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRH2502

   Plasmid

   #84379

   Purpose

   Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   dcas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Aspartate at position 10 changed to Alanine, Hist…

   Promoter

   uv15tetO

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-zDjCas13d-His

   Plasmid

   #234481

   Purpose

   Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DjCas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET32a-HD46Q

   Plasmid

   #11515

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 46Q glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 46 glutamines, vector m…

   Promoter

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. keratin 8 (krt8)-H2B-RFP

   Plasmid

   #105955

   Purpose

   transgenesis

   Depositor

   Caren Norden

   Article

   Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

   Insert

   krt8 (KRT8 Human)
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEN-CoV2-nanobody-F1

   Plasmid

   #194592

   Purpose

   Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   F1 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHis17_ParM _2

   Plasmid

   #78202

   Purpose

   Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

   Insert

   parM (K33A D63C T174A T175N D224C C287A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A D63C T174A T175N D224C C287A

   Promoter

   unknown

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSET-iGluSnFR4f

    Plasmid

    #234444

    Purpose

    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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