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R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159546

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET-21a-PE2-His

Plasmid

#201973

Purpose

Express His-tagged PE2 in bacteria

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

PE2-HIS

Tags

His and c-myc NLS

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJ1996_v2

Plasmid

#140664

Purpose

dCas9 & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9 & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCm C-term MBP NikJC199U

Plasmid

#174365

Purpose

Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis, MBP, and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCgsgRNA_crtYf

Plasmid

#153338

Purpose

Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

Insert

crRNA targeting crtYf

Use

CRISPR

Expression

Bacterial

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-Tn7-Pcym

Plasmid

#225280

Purpose

Mini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.

Depositor

Mark Schembri

Article

Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

Type

Empty backbone

Expression

Bacterial

Promoter

Pcym cumate (p-isopropylbenzoate)-inducible promo…

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAD-LyseR (in lacZ deficient strain)

Plasmid

#99245

Purpose

For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Expression

Bacterial

Promoter

beta-lactamase promoter

Available Since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmRE-Tn5-147

Plasmid

#118526

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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BB-NIC-II-HisN-TET12

Plasmid

#45496

Depositor

Roman Jerala

Article

Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

Insert

TET12

Use

Synthetic Biology

Tags

6xHistidine tag

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pG1AK-mCherry

Plasmid

#71737

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

mCherry

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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AWP-029

Plasmid

#213966

Purpose

Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

Depositor

Alexander Westermann

Article

Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

Insert

Cas12a (cas12a Segatella bryantii, Synthetic)

Tags

3XFLAG

Expression

Bacterial

Mutation

D875A to inactivate target nuclease activity

Promoter

PcfxA, IPTG inducible

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pExp-xMBP-ACE2-CHis

Plasmid

#194998

Purpose

Production of ACE2 extracellular domain

Depositor

Marko Hyvönen

Article

Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

Insert

ACE2 (ACE2 Human)

Tags

His8 and xMBP

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pRADZ1

Plasmid

#47698

Purpose

General cloning vector for use in E. coli or D. radiodurans with lacZ

Depositor

Mary Lidstrom

Article

Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

Type

Empty backbone

Expression

Bacterial

Promoter

repU

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLK1_Pkinase

Plasmid

#109933

Purpose

Protein expression and purification of PLK1_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLK1_Pkinase

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLG004 (RT-Abi-P2)

Plasmid

#157882

Purpose

Expresses the RT-Abi-P2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

RT-Abi-P2

Expression

Bacterial

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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