32,115 results
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Plasmid#209976PurposeContains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (SH71_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNCS mRFP
Plasmid#91765PurposeBacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)DepositorInsertmonomeric Red Fluorescent Protein1
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mHoneydew
Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
Tagsno tagsExpressionBacterialPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TetR/pTet-Tnpase (camR)
Plasmid#213910PurposeATc-inducible himar1C9 transposase expression - cam resistanceDepositorInsertTetR/ptet ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXT129_TwinStrep-SUMO-ShTnsB
Plasmid#135525PurposeProtein expression construct for ShTnsBDepositorInsertShTnsB
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
ExpressionBacterialPromoterpBADAvailable SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
LB1461 (Bcl-2 promoter with MHS1234, NFkB mut)
Plasmid#15385DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS123…Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
TagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
ExpressionBacterialMutationNahR: I25V P72H V118A R248CAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-SMAD4
Plasmid#16491DepositorAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
human p53-(94-312)
Plasmid#24866DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-mCherry
Plasmid#66533PurposeExpresses mCherry under the control of Mutant T7 Promoter G6DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - G6Available SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
ExpressionBacterialPromoterN/AAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
19 pCOLI_A_SUMO
Plasmid#160257PurposeN-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and SUMOExpressionBacterialPromoterT7Available SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only