29,771 results

1. pCAG-PuroDTK.Neo

   Plasmid

   #72307

   Purpose

   PiggyBac Cassette contains puromycin and TK genes

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

   Insert

   Puromycin-DTK-neo
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Feb. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR-Hyg

   Plasmid

   #158708

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Hsp60

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. HSA1_His

   Plasmid

   #202763

   Purpose

   human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA1
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP223

   Plasmid

   #13263

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13. 4():11.

   Insert

   Plac-gam-bet-exo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-X(5010)B1

   Plasmid

   #168019

   Purpose

   Expresses X(5010)B1-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   X(5010)B1-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMEG_YLCas9_empty

   Plasmid

   #117826

   Purpose

   CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Insert

   YlpTEF_hCas9_YlCyc1TT
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYTK080

   Plasmid

   #65187

   Purpose

   Encodes ZeocinR as a Type 6 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   ZeocinR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. SRPK1 (SRPK1A-c100)

   Plasmid

   #98246

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SRPK1A (SRPK1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. "AND-gate" receiver plasmid

   Plasmid

   #127676

   Purpose

   Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

   Insert

   LuxR, GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 for LuxR, pLux for GFP

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL-c5x_UnaG

    Plasmid

    #163125

    Purpose

    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    UnaG
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1A

    Plasmid

    #162692

    Purpose

    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T1-USP11

    Plasmid

    #78849

    Purpose

    To purify GST-tagged recombinant USP11 protein

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

    Insert

    USP11 (USP11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

14. TDP-43_CTD_M337V

    Plasmid

    #98674

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M337V

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTPH413

    Plasmid

    #185728

    Purpose

    Bacterial PAM profiling of adenine base editor variants

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Inserts

    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEB2-DsRed-Express2

    Plasmid

    #103999

    Purpose

    Plasmid encoding DsRed-Express2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    DsRed-Express2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. IL2 pMalLP

    Plasmid

    #41808

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Use

    Tags

    3C protease site, 6x His, and Maltose Binding Pro…

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Jan. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. RHOAA-c033

    Plasmid

    #205116

    Purpose

    RHOA protein expression

    Depositor

    Paul Brennan

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSHDY-PL03-mVenus_tetR

    Plasmid

    #137663

    Purpose

    Conjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.

    Depositor

    Ilka Axmann

    Article

    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

    Insert

    PL03:mVenus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL03

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-6p1-GST-ArcΔCTD

    Plasmid

    #119878

    Purpose

    Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of nt 832-1110

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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