33,449 results
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Plasmid#91761PurposeBacterial expression of a fluorescent protein, CitrineDepositorInsertCitrine
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PhoCl
Plasmid#87688PurposeExpress PhoCl in E. coliDepositorInsertPhoCl
Tags6xHisExpressionBacterialAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB094a
Plasmid#79241PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4; PC araC
ExpressionBacterialPromoterPBAD; PCAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ubiquitin K63C
Plasmid#86589PurposeBacterial expression of Ubiquitin K63C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
TagsHis6-MBPExpressionBacterialMutationTEV site; K63CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
RanBP2-RB3-4-pET23a
Plasmid#85769Purposebacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tagDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsHisExpressionBacterialMutationfragment (amino acids 2304-3062)PromoterT7Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 AC30_eGFP
Plasmid#82715PurposeExpresses a GFP-tagged recombinant adenylyl cyclase.DepositorTagsFLAG purification tag and eGFPExpressionBacterial and InsectPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCis GFPuv-SS Himar1 A7
Plasmid#84686PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-Rpl10a
Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
TagsAviExpressionBacterialPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET Cas1 Cas2
Plasmid#80088PurposeEncodes Cas1 and Cas2DepositorPublicationAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCOLA-T7-YhjH
Plasmid#79165PurposeExpresses YhjH under a T7 promoterDepositorInsertyhjH
ExpressionBacterialPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET31b-T7-Spinach2-Ct
Plasmid#79158PurposeExpresses the Ct biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Ct
ExpressionBacterialPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa
Plasmid#78182PurposeExpresses ffDronpa E. coli strainsDepositorInsertffDronpa
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterChicken B-ActinAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorPublicationInsertsgRNA rfp (T)
UseSynthetic BiologyExpressionBacterialPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only