30,320 results

1. pOPINK-OTUD1 (full-length, aa 1-481)

   Plasmid

   #61405

   Purpose

   Expresses human OTUD1 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCKan-phoAp-GFPuv

   Plasmid

   #139072

   Purpose

   Low phosphate inducible promoter phoAp for GFP expression

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

   Insert

   phoAp-GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKESaPar

   Plasmid

   #211829

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-3'TwStrep

   Plasmid

   #208053

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   Twin Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBFC0996

   Plasmid

   #186695

   Purpose

   VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   crRNA
   Tn7 transposon
   2xLguI Cargo Stuffer
   SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2attB-FST344-HA-CPL

   Plasmid

   #131460

   Purpose

   Encodes human Follistatin protein

   Depositor

   Michele Calos

   Article

   Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

   Insert

   Follistatin (FST Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAT206

   Plasmid

   #134883

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBbB8k-GFP

   Plasmid

   #35363

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BAD

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-Dynamin 2 K44A

   Plasmid

   #22301

   Depositor

   Pietro De Camilli

   Article

   Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

   Insert

   Dynamin 2 K44A (Dnm2 Rat)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   K44A

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pSEVA228-pro4IUPi

    Plasmid

    #122018

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pro4

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZS47a luxI-laa RBS

    Plasmid

    #48892

    Purpose

    ArsR-regulated expression of luxI-laa

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pArsR

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIND

    Plasmid

    #24730

    Depositor

    Brian Robertson

    Article

    Blokpoel et al Nucleic Acids Res. 2005 . 33(2):e22.

    Insert

    TetRO promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-tdTomato-hphMX6

    Plasmid

    #105157

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

    Insert

    tdTomato-hphMX6
    

    Use

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-11-LECT2-I40

    Plasmid

    #183032

    Purpose

    Expression of LECT2 I40 variant protein from T7 promoter

    Depositor

    Gareth Morgan

    Article

    Belonogov et al bioRxiv 2022.03.01.482540

    Insert

    LECT2 (LECT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I40 variant

    Promoter

    T7

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFTK055

    Plasmid

    #171327

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    rTA2S-M2 (TetR-3xVP16) transcriptional activator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIOM15

    Plasmid

    #20869

    Depositor

    Carmen Beuzón

    Article

    Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

    Insert

    aphA::aadA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

19. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDSG360

    Plasmid

    #115601

    Purpose

    surface-displayed Ag3; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge