30,803 results

1. pTXTL-T7max-NanoLuc

   Plasmid

   #188313

   Purpose

   Expression of NanoLuc via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   NanoLuc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZa pBAD csgA CBDcex pLac Ara csgGEF

   Plasmid

   #226378

   Purpose

   Plasmid carries csgA and csgG,E,F genes of curli operon

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   csgA and csgG,E,F genes of curli operon
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbS8c-ddcpf1-rfp

   Plasmid

   #153038

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Expression

   Bacterial

   Mutation

   E993A

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-meGFP-DLC1

   Plasmid

   #192048

   Purpose

   Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

   Depositor

   Ionel Popa

   Article

   Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

   Insert

   DLC1 (DLC1 Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETA-qmTQ2-ATP

   Plasmid

   #225247

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-ATP
   

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-GFP-Rab27a

   Plasmid

   #194985

   Purpose

   overexpress Rab27a

   Depositor

   Angelique Bordey

   Insert

   GFP-Rab27a
   

   Expression

   Bacterial

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pELlR4

   Plasmid

   #172545

   Purpose

   Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Carbenicillin Resistance Marker.

   Depositor

   Christopher Reisch

   Insert

   LacI-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLlacO-1

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24a-LaM4-EGFP

   Plasmid

   #182641

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
   

   Tags

   BAP, EGFP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGDP3 arr

   Plasmid

   #112890

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   arr
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-HisD-miRFP713-HO1

    Plasmid

    #197255

    Purpose

    Expresses the protein of miRFP713 and HO1 in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP713
    

    Expression

    Bacterial

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-tdMCP-msfGFP(V206K)-GBI

    Plasmid

    #205163

    Purpose

    express tdMCP-msfGFP(V206K)-GBI

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    tdMCP-msfGFP(V206K)-GBI
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQLinkG2

    Plasmid

    #13671

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

    Insert

    multi cloning site
    

    Use

    Co-expression

    Tags

    GST, TEV, and attB1

    Expression

    Bacterial

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX4T3-HA-ERK2 GOF

    Plasmid

    #53200

    Purpose

    Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    R67S/D321N

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pFA6a- mEGFP-kanMX6

    Plasmid

    #105146

    Purpose

    Yeast gene targeting

    Depositor

    Thomas Pollard, Jian-Qiu Wu

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    mEGFP-kanMX6
    

    Tags

    mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTECH-MmAcK3RS(IPYE)

    Plasmid

    #104071

    Purpose

    Expression plasmid encoding MmAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmAcK3RS(IPYE); PproK pyl
    

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid

    #177722

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Tags

    GFP11, HA, KDEL, and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJ2072.2_c1

    Plasmid

    #140675

    Purpose

    CRISPRlator control (“open ring”)

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    CRISPRlator control (“open ring”)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTmpKm

    Plasmid

    #176227

    Purpose

    A template plasmid for amplification of the cI-hok-neo cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, neo
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBY3_ligD-HygR

    Plasmid

    #104625

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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