30,535 results

1. pUCP30T-E2Crimson

   Plasmid

   #78478

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

   Plasmid

   #164615

   Purpose

   Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

   Depositor

   Jeffrey Chao

   Article

   Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xMS2v5 (3'UTR) and SunTag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNA2.0 6H-TEV-KRAS

   Plasmid

   #111849

   Purpose

   Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

   Depositor

   Kenneth Westover

   Article

   Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

   Insert

   KRAS (KRAS Human)
   

   Use

   Tags

   6xHis-tag and TEV protease cleavage sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTOX5

   Plasmid

   #127450

   Purpose

   Encodes MqsR toxin and amilCP chromoprotein. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Inserts

   mqsR toxin
   amilCP chromoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized from protein sequence in FPbase

   Promoter

   pRha and tac

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE30-His-CKM

   Plasmid

   #124134

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Creatine kinase (CKM Chicken)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ELONGIN BC (XX01TCEB1A-c001)

   Plasmid

   #110274

   Purpose

   Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

   Depositor

   Nicola Burgess-Brown

   Insert

   Elongin BC (ELOC, ELOB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Elongin B is full length. Elongin C contains a.a…

   Promoter

   T7

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC1-human APPL1

   Plasmid

   #22198

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET/ELP-I-CAT

   Plasmid

   #71461

   Purpose

   ELP-intein purification system

   Depositor

   David Wood

   Article

   Banki et al Nat Methods. 2005 Sep;2(9):659-61.

   Type

   Empty backbone

   Use

   Tags

   ELP ((VPGXG)110) and intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-N-FLAG

   Plasmid

   #119759

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Use

   Tags

   FLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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