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pUC19_MCM2_dTAG

Plasmid

#232731

Purpose

Generates MCM2 dTAG C ternimal knock-in cell lines as donor

Depositor

Junjie Chen

Insert

MCM2 (MCM2 Human)

Expression

Bacterial

Mutation

Synonymous mutation

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBSMrnaSeph

Plasmid

#67507

Purpose

Express the tRNAMet scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pSR1037

Plasmid

#208736

Purpose

Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PSarA1

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GST HP1 beta

Plasmid

#24075

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 beta (CBX1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pUC18-mini-Tn7T

Plasmid

#64954

Purpose

mini-Tn7 base vector with transcriptional terminator

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCoofy44

Plasmid

#55185

Purpose

Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

Expression

Bacterial

Promoter

pPH (Polyhedrin promoter)

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-Sth1Cas9

Plasmid

#129552

Purpose

Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

Sth1Cas9

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNCS tdTomato

Plasmid

#91767

Purpose

Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

tandem dimer Tomato

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_HA

Plasmid

#184866

Purpose

pSwap plasmid part with HA homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP WT

Plasmid

#197575

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6)

Tags

His6

Expression

Bacterial

Promoter

araC and lpp

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_PRKACB_Pkinase

Plasmid

#110024

Purpose

Protein expression and purification of PRKACB_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACB_Pkinase (PRKACB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-NanoLuc

Plasmid

#188313

Purpose

Expression of NanoLuc via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

NanoLuc

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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GST-mCh-FYVE

Plasmid

#192289

Purpose

Expresses GST- and mCherry-tagged HRS FYVE in e Coli

Depositor

James Hurley

Article

Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

Insert

HRS FYVE domain

Tags

GST-TEV-mCherry

Expression

Bacterial

Promoter

tac

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Expression

Bacterial

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET_GFP-SZ2

Plasmid

#124931

Purpose

Expression of GFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

GFP-SYNZIP2

Tags

GFP and His

Expression

Bacterial

Mutation

SZ2 domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET24b-Amg2

Plasmid

#199434

Purpose

expresses Amg2 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

Amg2

Tags

his tag

Expression

Bacterial

Mutation

L159K mutation

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS(B)-Rheb-Q64L

Plasmid

#21050

Depositor

Peter Vogt

Article

Jiang et al Oncogene. 2008 Sep 25. 27(43):5729-40.

Insert

Ras-homolog enriched in brain (RHEB Human)

Use

Retroviral

Tags

FLAG

Expression

Bacterial

Mutation

glutamine 64 to leucine

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

Plasmid

#30505

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Tags

His

Expression

Bacterial

Available Since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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