30,803 results

1. pEB1-mVenus ME

   Plasmid

   #103989

   Purpose

   Plasmid encoding mVenus ME

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mVenus ME
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F46L, F64L, …

   Promoter

   proC

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDule-para-aminoPhe

   Plasmid

   #85502

   Purpose

   Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

   Insert

   p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32T, E107T, D158P, I159L, L162A

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-HsSENP3

   Plasmid

   #71467

   Purpose

   Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG327

   Plasmid

   #128797

   Purpose

   Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2 (NEWENTRY Synthetic)
   

   Expression

   Bacterial

   Promoter

   Pxyl(s)

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-XI

   Plasmid

   #157988

   Purpose

   Expresses XI in E.coli cell

   Depositor

   Byung Il Lee

   Insert

   xylose isomerase
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET_BGL3

   Plasmid

   #101912

   Purpose

   Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.

   Depositor

   Steve Shih

   Article

   Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

   Insert

   β-glucosidase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7+LacO

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC575

   Plasmid

   #127976

   Purpose

   AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   ble (bleomycin resistance marker)
   

   Use

   Fungal expression

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans trpC promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p11d-aRPA (MSW#712)

   Plasmid

   #208078

   Purpose

   Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

   Depositor

   Marc Wold

   Article

   Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

   Insert

   RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-P70a-3WJdB-T

    Plasmid

    #87311

    Purpose

    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Three-way Junction dimeric Broccoli aptamer
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAGGS-EGFP-NLS-3XFLAG-V5

    Plasmid

    #196090

    Purpose

    Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    GFP
    

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Promoter

    CAGGs

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG03

    Plasmid

    #154138

    Purpose

    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

    Depositor

    Kang Zhou

    Article

    Panda et al Biochemical Engineering Journal

    Insert

    fucO, aldA
    

    Expression

    Bacterial

    Promoter

    P_gyrA

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDSG398

    Plasmid

    #115604

    Purpose

    surface-displayed Nb3; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-C2-SNAP

    Plasmid

    #208769

    Purpose

    To express C2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Tags

    SNAP-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Spacer Switch Plasmid

    Plasmid

    #215346

    Purpose

    Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    OpaD spacer; Chloramphenicol Resistance Cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSMART-HCKan-yibDp-VHH-VCAM1

    Plasmid

    #202471

    Purpose

    Expresses the nanobody VCAM1 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VCAM1
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pzntA_LacZ

    Plasmid

    #216325

    Purpose

    PZntA promoter driving expression of lacZ

    Depositor

    Mark Styczynski

    Article

    McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

    Insert

    b-Galactosidase
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMMLV-mRFP1-P2A-Puro

    Plasmid

    #160228

    Purpose

    Expresses mRFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    mRFP1-P2A-Puro
    

    Use

    Retroviral

    Expression

    Bacterial and Mammalian

    Promoter

    LTR

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-PIPK1 gamma 87

    Plasmid

    #22300

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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