30,296 results

1. pHis-parallel1-NLSH2BCas9

   Plasmid

   #112065

   Purpose

   Expression and purification of NLSH2BCas9 for fungal gene editing

   Depositor

   Jeff Coleman

   Article

   Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

   Insert

   NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
   

   Use

   CRISPR

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Cas9 was codon optimized for Aspergillus niger

   Promoter

   promoter in vector

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBS-HxB.S

   Plasmid

   #65415

   Purpose

   contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

   Depositor

   Harold Erickson

   Article

   Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

   Insert

   tenascin C small splice variant (TNC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103_EEF1A1_GTP-EFTU

   Plasmid

   #109861

   Purpose

   Protein expression and purification of EEF1A1_GTP-EFTU

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   EEF1A1_GTP-EFTU (EEF1A1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. APRT

   Plasmid

   #64028

   Purpose

   Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.

   Depositor

   Vern Schramm

   Article

   Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9

   Insert

   adenine phosphoribosyltransferase (APT1 Budding Yeast)
   

   Use

   Tags

   Histidine x 6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCR8-attL5-pegRNA-attR3

   Plasmid

   #213054

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGJJ001

   Plasmid

   #183619

   Purpose

   bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

   Depositor

   Ben Lehner

   Article

   Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. N-WASP

   Plasmid

   #186132

   Purpose

   For expression of recombinant N-WASp in bacteria cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   WASL (WASL Rat)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Amino Acid residues 183-193 fused to 273-501

   Promoter

   T7

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. His_CL7_2NLS_iCas12a-4NLS

   Plasmid

   #222971

   Purpose

   Bacterial expression of NLS-rich iCas12a construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His_CL7_2NLS_iCas12a-4NLS
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDD57

   Plasmid

   #119882

   Purpose

   Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pConstitutive_GFP+
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pScI_dCas9-CDA

    Plasmid

    #108549

    Purpose

    Bacterial Target-AID vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-mH6-Cas12c2

    Plasmid

    #120873

    Purpose

    Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET_RGG

    Plasmid

    #124929

    Purpose

    Expression of RGG domain from LAF-1 gene

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    LAF-1 RGG domain (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    RGG domain

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NEDD4

    Plasmid

    #25141

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    nedd4.0001.0152 (NEDD4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pET28a-MSP1D1delltaH4

    Plasmid

    #71713

    Purpose

    Membrane scaffold protein (MSP), helix 4 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBA562

    Plasmid

    #186237

    Purpose

    pTet-RfxCas13d crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYH78

    Plasmid

    #217386

    Purpose

    Ptrc99A-PAmCherry for bacterial expression

    Depositor

    Anthony Vecchiarelli

    Article

    Hoang et al Nat Commun. 2024 Apr 15;15(1):3222. doi:

    Insert

    PAmCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hDHFR

    Plasmid

    #184707

    Purpose

    bacterial expression of human DHFR

    Depositor

    Peter Wright

    Article

    Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

    Insert

    DHFR (DHFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-mOct4 Full length(FL)

    Plasmid

    #206394

    Purpose

    Expresses Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C1-62 pComb3xss anti-cFos nanobody

    Plasmid

    #145835

    Purpose

    Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-cFos (Human) recombinant llama nanobody C1-62 (FOS L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-32a(+)-TGFB1

    Plasmid

    #120361

    Purpose

    Produce human recombinant TGFB1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β1 (TGFB1 Human, Synthetic)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge