30,803 results

1. p2H12Matry

   Plasmid

   #221143

   Purpose

   cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen-Matry
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1delZn2

   Plasmid

   #173935

   Purpose

   Bacterial expression of PARP1 lacking Zn2 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn2 (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 97-213 (V762A)

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDFDuet-nsp7-nsp8

   Plasmid

   #159092

   Purpose

   Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

   Depositor

   Seth Darst

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Inserts

   NSP7
   NSP8
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15-ZmPYK

   Plasmid

   #220230

   Purpose

   Inducible expression of Zymomonas mobilis pyruvate kinase

   Depositor

   Aron Fenton, Liskin Swint-Kruse

   Article

   Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

   Insert

   Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b-Caspase-3(V266E)

   Plasmid

   #90089

   Purpose

   Expresses caspase 3 V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   V266E

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

   Plasmid

   #73404

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   PhCHS
   CmCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7_zntR

   Plasmid

   #216324

   Purpose

   T7 promoter driving expression of zntR

   Depositor

   Mark Styczynski

   Article

   McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

   Insert

   Zinc-responsive activator
   

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET28a SUMO Nup53xl 1-320

   Plasmid

   #85998

   Purpose

   bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   Xenopus laevis Nup53 (nup35.L Frog)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLlacO-1-mfLon

   Plasmid

   #45152

   Depositor

   Michel Maharbiz

   Article

   Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

   Insert

   Lon
   

   Use

   Synthetic Biology

   Tags

   ssRA degradation (LVA)

   Expression

   Bacterial

   Promoter

   PLlacO-1

   Available Since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLS.402

    Plasmid

    #187202

    Purpose

    Expresses retron Eco1 RT

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    Retron Eco1 Reverse Transcriptase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    mphR

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-CRY2Clust

    Plasmid

    #200969

    Purpose

    induction of phase-separated condensate by CRY2Clust

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    CRY2Clust
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. His SUMO TEV WT SPOP 28-359

    Plasmid

    #194115

    Purpose

    Wild type His-SUMO SPOP expression plasmid

    Depositor

    Tanja Mittag

    Article

    Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

    Insert

    SPOP (SPOP Human)
    

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-21a_FnCpf1_2xNLS_protein_expression

    Plasmid

    #124548

    Purpose

    Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnCpf1_2xNLS
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-Cdk9 T186A

    Plasmid

    #28101

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Contains a T186A mutation in Cdk9

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

    Plasmid

    #48308

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMal_Abl-PRM 3R

    Plasmid

    #112086

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    Abl-PRM-3R (ABL1 Human)
    

    Tags

    His-6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only the PRM motif of Abl1

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHV739

    Plasmid

    #117477

    Purpose

    Expression of NMT2

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    NMT2 (NMT2 Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

    Plasmid

    #119061

    Purpose

    Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    pax3a_enh5-E1b:eGFP
    

    Expression

    Bacterial

    Promoter

    E1b minimal promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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