29,776 results

1. pECJ3

   Plasmid

   #75465

   Purpose

   Toggle switch plasmid containing lacI-pdt3

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   lacI-pdt3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST17-UbE2T

   Plasmid

   #15808

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2T (UBE2T Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pBbdCas9S(RBSmut)_Psyn-sgRNA500

   Plasmid

   #149576

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAP01

   Plasmid

   #59981

   Purpose

   The function of this material is to facilitate the capture and expression of bacterial gene clusters.

   Depositor

   Bradley Moore

   Article

   Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

   Type

   Empty backbone

   Use

   Synthetic Biology; Transformation-associated reco…

   Tags

   no

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   no

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNR64

   Plasmid

   #79540

   Purpose

   Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   

   Use

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TurboRFP-pBAD

   Plasmid

   #54858

   Purpose

   Localization: Protein Expression Vector , Excitation: 553, Emission: 574

   Depositor

   Dmitriy Chudakov, Michael Davidson, Dorus Gadella

   Article

   Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

   Type

   Empty backbone

   Use

   Tags

   6xHis and TurboRFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pGEX Src-SH2

   Plasmid

   #46510

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Src (SRC Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 142-252

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNIC-ZB

   Plasmid

   #26107

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Type

   Empty backbone

   Use

   Tags

   His6 - Z-basic - TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NpuDnaEc-cGCaMP2

   Plasmid

   #34851

   Depositor

   Kevin Truong

   Article

   Wong et al Cell Calcium. 2011 Nov 29.

   Insert

   NpuDnaEc-cGCaMP2
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV, T7 lac

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Q-IL6-9

    Plasmid

    #166552

    Purpose

    scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMH610

    Plasmid

    #131862

    Purpose

    Produces holo-PhyB* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    PhyB(1-651)-RGD-His6-Cys
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFGM1

    Plasmid

    #64949

    Purpose

    source of GmR FRT cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    FRT-GmR-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DUSP13

    Plasmid

    #38824

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DUSP13 (Dusp13 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET21a-hAIM2-His6

    Plasmid

    #51532

    Purpose

    Express His6-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCK01_PalkS_alkS

    Plasmid

    #166502

    Purpose

    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    alkS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    L401G

    Promoter

    PalkS

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAB_PalkB_sfgfp_KanR

    Plasmid

    #166503

    Purpose

    Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PalkB

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEND-193_Cacnes_HS

    Plasmid

    #225622

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEND-229_Cacnes_EforCP

    Plasmid

    #225615

    Purpose

    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    EforCP
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEND-164_Cacnes_pFAST

    Plasmid

    #225616

    Purpose

    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    pFAST
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge