30,803 results

1. pSF-p15A-trc- YFP

   Plasmid

   #186569

   Purpose

   p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Expression

   Bacterial

   Promoter

   trc promoter

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEcRNAP6

   Plasmid

   #128940

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoA (rpoA E. coli)
   RpoB
   RpoC-ppx(His)10
   RpoZ
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   PreScission protease site (GE Healthcare; LEVLFQG…

   Promoter

   T7

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Dynamin 2 K44A

   Plasmid

   #22301

   Depositor

   Pietro De Camilli

   Article

   Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

   Insert

   Dynamin 2 K44A (Dnm2 Rat)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   K44A

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pET 2C-T10-dCasX

   Plasmid

   #126418

   Purpose

   E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   His-MBP

   Expression

   Bacterial

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGWB402-er-mCherry

   Plasmid

   #186288

   Purpose

   Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   er-mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pROP-IP-GFP

   Plasmid

   #122136

   Purpose

   GFP Reporter plasmid for B-Rex contain imperfect operator sequence

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   GFP
   

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET151-GyrB

   Plasmid

   #216233

   Purpose

   S. aureus USA300 GyrB Codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. bactin:GFP-LAP2b

   Plasmid

   #105980

   Purpose

   transgenesis, ubiquitous expression

   Depositor

   Caren Norden

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   LAP2b
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJL1-mRFP1

   Plasmid

   #102630

   Purpose

   In vitro expression of mRFP1 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mRFP1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbdCas9S_Psyn-sgRNA500

    Plasmid

    #149640

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcI-ts,ind+-(NSP8)

    Plasmid

    #160656

    Purpose

    Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

    Insert

    SARS-CoV-2 NSP8 (ORF1ab Synthetic)
    

    Expression

    Bacterial

    Available Since

    Dec. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET-24a-ELP[V-150]

    Plasmid

    #67015

    Purpose

    Expresses ELP[V-150] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    ELP[V-150]
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSG360zeo

    Plasmid

    #27154

    Depositor

    Michael Glickman

    Article

    Barkan et al J Bacteriol. 2010 Jul . 192(14):3661-8.

    Type

    Empty backbone

    Use

    Mycobacterial ko vector

    Expression

    Bacterial

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. NADK

    Plasmid

    #28268

    Depositor

    Cheryl Arrowsmith

    Insert

    NADK:HPC0AC-F12:C216828 (NADK Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains residues 68-426.

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pET29b-pduCDE

    Plasmid

    #110138

    Purpose

    Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

    Insert

    pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrc-GWS1B

    Plasmid

    #239582

    Purpose

    High copy vector for expression of GWS1B Rubisco

    Depositor

    Matthew D. Shoulders

    Insert

    GWS1B Rubisco (6X his)
    

    Tags

    6X histag

    Expression

    Bacterial

    Mutation

    Wild-type

    Promoter

    Trc

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBCPB+

    Plasmid

    #18940

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    attP, lacZ, attB
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. PAJ698

    Plasmid

    #178947

    Purpose

    Expresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)

    Depositor

    Alfonso Jaramillo

    Article

    Cunliffe et al ACS Synth Biol. 2022 Oct 21;11(10):3207-

    Insert

    Chimeric Tail Fiber Fusion P2-gpH and S16 gp37
    

    Use

    Unspecified

    Expression

    Bacterial

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDM_T7_HisLacZomega

    Plasmid

    #118815

    Purpose

    T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    lacZ_
    

    Tags

    histag

    Expression

    Bacterial

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-20b-M-Ntx-S1

    Plasmid

    #58716

    Purpose

    Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Changed Asparagine 1 to Serine

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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