29,776 results

1. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Guillermo et al Cell Systems, 2025

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPTI-[5,55]Gly14Ile38

   Plasmid

   #195059

   Purpose

   Thermal stability of BPTI

   Depositor

   Yutaka Kuroda

   Article

   Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

   Insert

   bovine pancreatic trypsin inhibitor
   

   Use

   Tags

   His tag and trpΔLE leader sequences

   Expression

   Bacterial

   Mutation

   Changed Alanine residues at position of 14 and 38…

   Promoter

   T7 promotor

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSR:MSMEG_0835:Dendra

   Plasmid

   #174923

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0835
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST17-UbE2R2

   Plasmid

   #15806

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG020 (AVAST type 4)

   Plasmid

   #157898

   Purpose

   Expresses the AVAST type 4 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   AVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG019 (AVAST type 3)

   Plasmid

   #157897

   Purpose

   Expresses the AVAST type 3 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   AVAST type 3 (STAND ATPase with N-terminal DUF4297)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECGMC3

   Plasmid

   #75441

   Purpose

   Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   mf-lon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   nisA promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET Flag TEV LIC cloning vector (1L)

   Plasmid

   #29662

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Flag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-2T-RhoA-T19N

   Plasmid

   #12960

   Depositor

   Gary Bokoch

   Insert

   RhoA DN (RHOA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T19N Dominant negative

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEVA221rAPOBEC1-T7RNAP

    Plasmid

    #167979

    Purpose

    Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    rAPOBEC1-T7RNAP (T7p07 Synthetic)
    

    Use

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b_Q69L

    Plasmid

    #186272

    Purpose

    Expression vector of His-RanQ69L in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Use

    Tags

    His-tagged at N terminal

    Expression

    Bacterial

    Mutation

    Changed Q69 into L

    Promoter

    T7 promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KLHL3 (KLHL3A-c003)

    Plasmid

    #110251

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL3 (KLHL3 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRBV28

    Plasmid

    #123458

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV28 (TRBV28 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRBV27

    Plasmid

    #123457

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV27 (TRBV27 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid

    #107292

    Purpose

    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-GFP (SNCA Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18T-mini-Tn7T-Zeo-dsRedExpress

    Plasmid

    #65040

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. 6BY9 (EHMT1)

    Plasmid

    #119956

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    EHMT1 (EHMT1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge