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pET51b(+)_LAMA-F98 (for GFP)

Plasmid

#130714

Purpose

Plasmid encoding the bacterial expression of LAMA-F98 for GFP

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-F98

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-6P-1-INTS3-FL

Plasmid

#128415

Purpose

Express INTS3 full-length in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

full-length

Promoter

tac promoter

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT7-L1T1L-NLuc

Plasmid

#128942

Purpose

Expresses L1-NLuc gene

Depositor

Takeshi Kobayashi

Publication

Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

Insert

L1 minor core protein

Tags

Nanoluc

Expression

Bacterial

Promoter

T7 promotor

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV801

Plasmid

#126044

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1

Plasmid

#128306

Purpose

Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBT13-splitC

Plasmid

#122596

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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OmpA-mNG-mCh

Plasmid

#124216

Purpose

Periplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh

Depositor

Tanneke den Blaauwen

Publication

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

OmpA-mNG-mCh

Expression

Bacterial

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah7

Plasmid

#122574

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah10

Plasmid

#122577

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah3

Plasmid

#122570

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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p15aC-GFP

Plasmid

#107862

Purpose

Control plasmid expressing GFP

Depositor

Sanford Simon

Publication

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

GFP

Tags

myc

Expression

Bacterial

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7xTS-sfGFP

Plasmid

#117390

Purpose

Tn7 tagging vector pTn7xTS with sfGFP expression scaffold

Depositor

Karen Guillemin

Publication

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-ArcΔCTD

Plasmid

#119878

Purpose

Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

Depositor

Jason Shepherd

Publication

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Tags

GST

Expression

Bacterial

Mutation

Deletion of nt 832-1110

Promoter

tac

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD011

Plasmid

#116852

Purpose

Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

Depositor

Friedrich Simmel

Publication

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
mScarletI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Publication

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Publication

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Publication

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6P1-N-Strep2

Plasmid

#119758

Purpose

Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.

Depositor

Andrew Jackson, Martin Reijns

Type

Empty backbone

Tags

GST and Strep-tag II fusion tag

Expression

Bacterial

Promoter

tac promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_45

Plasmid

#109243

Purpose

pSC101 var3 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1358

Plasmid

#119277

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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