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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Article

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_IRF3_SMAD/FHA

Plasmid

#178479

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

IRF3_IRF3 (IRF3 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBB572

Plasmid

#27397

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

ibpA, ibpB (ibpB, ibpA E.coli)

Expression

Bacterial

Available Since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

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pBbE6k-RFP

Plasmid

#35288

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Expression

Bacterial

Promoter

PBAD

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2

Plasmid

#113634

Purpose

An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

Depositor

Miguel Valvano

Article

Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

Type

Empty backbone

Expression

Bacterial

Promoter

PrhaB

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK5

Plasmid

#53166

Purpose

Expresses GST-HA tagged human MEK5 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pYPet-His

Plasmid

#14031

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Insert

YPet

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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