30,535 results

1. AASDHPPT

   Plasmid

   #39192

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AASDHPPT (AASDHPPT Human)
   

   Use

   Tags

   6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pRSET.HaloTag.v7.AviTag

   Plasmid

   #195486

   Purpose

   Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7.AviTag
   

   Use

   Tags

   6xHIS and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-KIRIN1-GR

   Plasmid

   #107417

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1-GR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. nifF in pET21b

   Plasmid

   #124520

   Purpose

   Azotobacter vinelandii flavodoxin II of Nitrogenase

   Depositor

   Douglas Rees

   Article

   Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

   Insert

   Flavodoxin II (nifF Azotobacter vinelandii)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / lac

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28GSTlinker-LIC

   Plasmid

   #119970

   Purpose

   Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCN-EF2132tet

   Plasmid

   #107191

   Purpose

   Expresses EF2132 recombinase for performing recombineering in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   EF2132
   tetR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p23

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

   Plasmid

   #169188

   Purpose

   To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE60-EFTs-His

   Plasmid

   #124128

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor Ts (tsf Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMART-SOS-GFPuv

   Plasmid

   #102283

   Purpose

   Fluorescent sensor of E. coli SOS response

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   cda-GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cda SOS promoter

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEND-164_Cacnes_pFAST

    Plasmid

    #225616

    Purpose

    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    pFAST
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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