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Plasmid EUPp_09

Plasmid

#157745

Purpose

PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

Depositor

Kang Zhou

Publication

Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

Inserts

acetaldehyde dehydrogenase
alcohol dehydrogenase (ADH2 Budding Yeast)

Expression

Bacterial

Promoter

PgyrA

Available since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-pelB

Plasmid

#157740

Purpose

empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites

Depositor

Debra Hansen

Type

Empty backbone

Tags

PelB signal sequence and possible His6-tag (adds …

Expression

Bacterial

Promoter

T7

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMS13: pTarget(AvCAST)

Plasmid

#168146

Purpose

Target plasmid containing AvPSP1 and attachement site for AvCAST.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PAM for AvCAST + AvPSP1
glmS

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His-SUMO-Rad23A_pET11a

Plasmid

#169124

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hRad23A (RAD23A Human)

Expression

Bacterial

Mutation

wt

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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His-SUMO-hDDI2_pET11a

Plasmid

#169120

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pM2s2AsR_TS-E10

Plasmid

#137944

Purpose

E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

Depositor

Neel Joshi

Publication

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Inserts

mRFP1
mWasabi

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101 and pTlpA-C7

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP

Plasmid

#166443

Purpose

Bacterial expression of 6x His tagged YAP

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP (YAP1 Human)

Expression

Bacterial

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-Optimized E6AP

Plasmid

#165100

Purpose

ecoli optimized expression of GST-fusion of human E6AP

Depositor

Arthur Haas

Publication

Ronchi et al J Biol Chem. 2013 Apr 12;288(15):10349-6

Insert

E6AP isoform 3 (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFLxR5

Plasmid

#149482

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKCyR8

Plasmid

#149494

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-mEGFP

Plasmid

#160464

Purpose

Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-mEGFP

Expression

Bacterial

Promoter

T5

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD_IPF2.0

Plasmid

#163124

Purpose

A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

Depositor

Julie Biteen

Publication

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

IFP2.0

Tags

His

Expression

Bacterial

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-RT006-GCN4-HaloPM

Plasmid

#163386

Purpose

To express 4X sGCN4 module in the G-baToN system

Depositor

Monte Winslow

Publication

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SNRPA_Full-length_pGEX

Plasmid

#135134

Purpose

Recombinant expression of SNRPA with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SNRPA (SNRPA Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Feb. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pYC1446

Plasmid

#158720

Purpose

Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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IGF2BP1_2xRRM-4xKH_pGEX

Plasmid

#135122

Purpose

Recombinant expression of IGF2BP1 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

IGF2BP1 (IGF2BP1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP5

Plasmid

#160088

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

Depositor

Pier Pandolfi

Publication

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG261-His-greenFAST

Plasmid

#160349

Purpose

Expresses His-tagged greenFAST in bacteria

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-greenFAST

Expression

Bacterial

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDest-521

Plasmid

#159688

Purpose

Gateway destination vector for E. coli protein expression

Depositor

Dominic Esposito

Publication

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Type

Empty backbone

Expression

Bacterial

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4B-pCMV7.1

Plasmid

#155023

Purpose

N-terminal 3xFLAG-tagged CUL4B in a pCMV7.1 vector

Depositor

Andre Catic

Publication

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4B (CUL4B Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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