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pLRD01

Plasmid

#208257

Purpose

Production of terpentetriene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cyc1
cyc2

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET30b_OmpAT7Sav

Plasmid

#138589

Purpose

T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

Insert

T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export

Use

Synthetic Biology

Tags

OmpA export signal and T7 tag

Expression

Bacterial

Promoter

PT7

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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BLMA-c800

Plasmid

#53631

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

BLMA

Tags

His-Tev

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
pD454-SR rat Alpha-synuclein

Plasmid

#89074

Purpose

bacterial expression of rat Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (Snca Rat)

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pMRE167

Plasmid

#118515

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pReporter_8

Plasmid

#60568

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_8

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Octo_VirD2_NLS-Cas9-6xHis

Plasmid

#232296

Purpose

Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

Depositor

Hee-Sik Kim

Article

Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

Octo_VirD2_NLS-Cas9-6xHis

Use

CRISPR

Tags

6xHis and Octopine-type VirD2-derived NLS

Expression

Bacterial

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-403-505

Plasmid

#165095

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTKIP-cat

Plasmid

#41065

Depositor

Edward Cox, Thomas Kuhlman

Article

Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

Plasmid

#159697

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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(SIM)10

Plasmid

#126946

Purpose

Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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IL13 promoter luciferase

Plasmid

#11784

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-13 promoter (IL13 Human)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only
pJBM4

Plasmid

#225291

Purpose

E. coli-Campylobacter shuttle vector; TetR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Tet(O) and its putative promoter

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Ki67∆FHA-mNeonGreen-IRESpuro2

Plasmid

#183739

Purpose

Expresses Ki67∆FHA-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67_lacking FHA (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67(lacking FHA)

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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plYJP022-int7

Plasmid

#202287

Purpose

Plasmid containing integrase for LPattB7 site

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

int7

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF-mTET1

Plasmid

#81052

Purpose

bacterial expression of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBsacA

Plasmid

#158650

Purpose

Constitutive transcription of sacA crRNA

Depositor

Wenjing Cui

Insert

sacA crRNA

Use

CRISPR and Synthetic Biology; Shuttle vector baci…

Expression

Bacterial

Promoter

Pveg

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Expression

Bacterial

Promoter

pTet

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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