30,535 results

1. pNHEJ-Cas9-recX

   Plasmid

   #158715

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTrc99S-ssDsbA-CRM197-4xDQNAT

   Plasmid

   #128403

   Purpose

   Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Diphtheria toxin
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating G52E mutation

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDCAF3-IS5

   Plasmid

   #65220

   Purpose

   Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

   Depositor

   Jeffrey Barrick

   Article

   Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

   Inserts

   ndmA
   ndmB
   ndmC
   ndmD
   gst9
   chlR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed start codon from gtg to atg and Changed s…

   Promoter

   BBa_J23100

   Available Since

   Jan. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRSET-RCaMP1h

   Plasmid

   #42874

   Purpose

   a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

   Depositor

   Loren Looger

   Article

   Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

   Insert

   Red Fluorescent Calcium binding Protein
   

   Use

   Tags

   EK (Enterokinase) Recognition Site, His Tag (6x),…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6His-MBP-MpAgo

   Plasmid

   #79249

   Purpose

   This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

   Depositor

   Jennifer Doudna

   Article

   Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

   Insert

   Marinitoga piezophila Argonaute
   

   Use

   CRISPR

   Tags

   6XHis-tag + MBP + PreScission Cleavage Site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCV020-tetQ

   Plasmid

   #232826

   Purpose

   Random transposon mutagenesis vector for Bacteroidales species - tetracycline

   Depositor

   Athanasios Typas

   Insert

   tetQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp-His6

   Plasmid

   #182386

   Purpose

   Bacterial expression of Vesicle Nucleating peptide

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmRFP_LAP2beta_IRES_puro2b

   Plasmid

   #21047

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   LAP2_beta (TMPO Rat)
   

   Use

   Tags

   mRFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB1A3_GFP_dT23

   Plasmid

   #89663

   Purpose

   expression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFP

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   GFP_dT23
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM5409

    Plasmid

    #132662

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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