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pHG4

Plasmid

#188252

Purpose

Integrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selection

Depositor

Hong Gao, Maggie Smith

Article

Gao et al BMC Biotechnol. 2019 Jun 4;19(1):32. doi

Type

Empty backbone

Expression

Bacterial

Promoter

act1p/actII-orf4

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500

Plasmid

#45788

Depositor

Richard Murray

Insert

deGFP

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

pBAD-tetO1

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Plasmid

#222991

Purpose

Bacterial expression of iGeoCas9(C2) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pN3

Plasmid

#233520

Purpose

Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARS

Depositor

Carl Denard

Type

Empty backbone

Expression

Bacterial and Yeast

Promoter

pGAL1

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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polySUMO1

Plasmid

#221066

Purpose

Expression of polySUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35S SUMO1

Plasmid

#221071

Purpose

Expression of poly H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35S

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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polySIM

Plasmid

#221068

Purpose

Expression of polySIM protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4

Tags

His and MBP

Expression

Bacterial

Promoter

unknown

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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polySUMO3

Plasmid

#221067

Purpose

Expression of polySUMO3 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of SUMO3 each separated by (GGS)4 (SUMO3 Human)

Tags

His and MBP

Expression

Bacterial

Promoter

tac

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:ChrH-ChrI

Plasmid

#225083

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-155

Plasmid

#185939

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-157

Plasmid

#185940

Purpose

Himar transposon delivery plasmid that delivers a green fluorescent mClover3 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mClover3

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-143

Plasmid

#185932

Purpose

Himar transposon delivery plasmid that delivers a yellow fluorescent sYFP2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

sYFP2

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pExodus CMV.Artemis

Plasmid

#40211

Purpose

Mammalian expression of codon-optimized Artemis.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Artemis (DCLRE1C Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pET28-AviTag-DogTag-MBP

Plasmid

#171773

Purpose

Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogTag-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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EPB41L3A-c000

Plasmid

#175458

Purpose

Protein expression in bacterial cells. Entire CDS, M1-D1087.

Depositor

Opher Gileadi

Insert

EPB41L3 (EPB41L3 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-msfGFP

Plasmid

#174492

Purpose

bacterial expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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WIW_Scaffold2

Plasmid

#205574

Purpose

Nanobody that inhibits seeding of tau fibrils

Depositor

David Eisenberg

Article

Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

WIW_Scaffold2

Tags

6X His

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiHistoneNb

Plasmid

#198293

Purpose

Produces PVCs retargeted with A9 anti-histone nanobody

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiHistoneNb

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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