33,123 results

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His6-SUMO-pET28a-EfaCas1

Plasmid

#112793

Purpose

To express enterococcus faecalis Cas1 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas1

Tags

His6-SUMO

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCON1-ProC.gfp

Plasmid

#112517

Purpose

ProC driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProC gfpmut3 TT

Expression

Bacterial

Promoter

ProC

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSW003

Plasmid

#111249

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBb(B5)8k-sfGFP

Plasmid

#110140

Purpose

IPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sfGFP

Expression

Bacterial

Available Since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-eyfp-a

Plasmid

#111620

Purpose

eYFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-eyfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B4-1

Plasmid

#111475

Purpose

Ala-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ala-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pQE32-pr-mEosFP (mEosFP-V69T)

Plasmid

#99213

Purpose

Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEosFP

Expression

Bacterial

Mutation

monomeric variant with mutation V69T for primed c…

Promoter

T5

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B1-2

Plasmid

#111464

Purpose

Met-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDL17

Plasmid

#106483

Purpose

pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

Depositor

Sergey Sineoky

Article

Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

Inserts

gam
bet
exo
lacI
rhaR
rhaS

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG_OriM

Plasmid

#110097

Purpose

Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Hyg)

Plasmid

#110077

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

Plasmid

#110572

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD-stRBS-aacC1

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTNwt-FL

Plasmid

#110217

Purpose

Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Expression

Bacterial

Mutation

P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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5T5G (SETD8)

Plasmid

#101886

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SETD8 (KMT5A Human)

Tags

6xHis, TEV

Expression

Bacterial

Promoter

T7

Available Since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pWT021a

Plasmid

#107895

Purpose

W2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT004a

Plasmid

#107883

Purpose

R1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PBAD-EGFP

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a- mYFP-kanMX6

Plasmid

#105147

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-kanMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mMaple3-kanMX6

Plasmid

#105165

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

mMaple3-kanMX6

Tags

GlySerGly-mMaple3

Expression

Bacterial and Yeast

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-566

Plasmid

#104195

Purpose

Expresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusion

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-566 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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