Skip to main content
Addgene
Showing: 5281 - 5300 of 29495 results

  1. pET-His6-Sumo-CfaC-MBP

    Plasmid
    #126018
    Purpose
    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)
    Depositor
    Thomas Muir
    Insert
    His6-Sumo-CfaC-MBP
    Use
    Tags
    6xHis-SUMO and HA-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126018-plasmid-map-sequence-id-248773

    Enlarge

  3. pDIV537

    Plasmid
    #177704
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177704-plasmid-map-sequence-id-352590

    Enlarge

  5. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

    Enlarge

  9. pBAD-MBP-His-SFP

    Plasmid
    #141141
    Purpose
    Expression of 4'-phosphopantetheinyl transferase gene
    Depositor
    New England Biolabs
    Insert
    4'-phosphopantetheinyl transferase
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141141-plasmid-map-sequence-id-298488

    Enlarge

  11. pQE30-rSyntenin1

    Plasmid
    #89428
    Purpose
    bacterial expression of rat Syntenin1
    Depositor
    Fritz Rathjen
    Insert
    SDCBP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89428-plasmid-map-sequence-id-177625

    Enlarge

  13. pBJ6

    Plasmid
    #167135
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Kanamycin promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167135-plasmid-map-sequence-id-330485

    Enlarge

  14. pCFJ1137

    Plasmid
    #84827
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Use
    Tags
    Expression
    Bacterial and Worm
    Mutation
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84827-plasmid-map-sequence-id-163671

    Enlarge

  18. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Use
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Promoter
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 5281 - 5300 of 29495 results