32,812 results
-
Plasmid#74746PurposepCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyExpressionBacterialPromoterpTacAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-S3
Plasmid#74693PurposeSrpR-S3 gateDepositorInsertPTac-RBSS3-SrpR-PSrpR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P3
Plasmid#74687PurposePhlF-P3 gateDepositorInsertPTac-RBSP3-PhlF-PPhlF-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLX4
Plasmid#73242PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TPH2
Plasmid#73243PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPIN4
Plasmid#73239PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAHA
Plasmid#74663PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_79 aka PAS620
Plasmid#71225PurposeReb_1 with RebA(Q91R)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA( G53A, Q91R)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_80 aka PAS621
Plasmid#71226PurposeReb_1 with RebB(I67T, N84I)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebB(I67T, N84I)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_65 aka PAS626
Plasmid#71231PurposeReb_1 with RebA(Y82A)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(G53A, Y82A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-T-HSV2.3 TCR alpha chain
Plasmid#74076PurposegBT-I TCR a chain cDNA in pGEM-TDepositorInsertTCR alpha clone HSV2.3 (Tcra Mouse)
ExpressionBacterialAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK234
Plasmid#73324PurposeProduces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioCDepositorInsertsviolacein biosynthesis protein VioA
violacein biosynthesis protein VioB
violacein biosynthesis protein VioC
ExpressionBacterialPromoterT7 and n/aAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4A6
Plasmid#73434PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.DepositorInsertRepressor 4A6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterpl-TetOAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK367
Plasmid#72406PurposeProduces Acetobacter aceti 1023 Est06DepositorInsertlipase
ExpressionBacterialMutationlacks Met1 and Thr2 in annotated (v1) sequencePromoterT7Available SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR6Kcat-rdxA-rpsL
Plasmid#45471DepositorInsertcat-rdxA-rpsL
ExpressionBacterialAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/prsA
Plasmid#73624PurposeProduces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tagDepositorInsertribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK225
Plasmid#71124PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coliDepositorInsertcitrate synthase
TagsHis6ExpressionBacterialPromoterT7Available SinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in VP2)
Plasmid#70032PurposepUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding regionDepositorInsertTSV full genome, linearized in VP2 gene
UseCloning vectorExpressionBacterialAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-STRNDHFR
Plasmid#63978PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTagsStrep II (STR); TEV cleavableExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKS913.CB18.31
Plasmid#69569PurposeTCR beta chain genomic transgenic construct for creating OT-1 miceDepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT588
Plasmid#68907PurposeNanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT937
Plasmid#68936PurposeRhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpRDepositorInsertInt12
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
4YWQ
Plasmid#69953PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRC3H1:YTC019-B07:C232791 (RC3H1 Human)
TagsHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable SinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-ARL8B
Plasmid#67334PurposehArl8b with stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SAR1
Plasmid#67344PurposehSar1 with stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only