33,123 results
-
Plasmid#90318PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only
-
pPBC-G6s
Plasmid#62812PurposepPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6s-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXY1
Plasmid#90487PurposePlasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
YeNL_pRSETb
Plasmid#89520PurposeYellow color variant of bright luminescent protein for bacterial expressionDepositorInsertYeNL
TagsHis X 6ExpressionBacterialPromoterT7Available SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(YF)-mCherry
Plasmid#87357PurposePI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutationDepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationY535FPromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD434-SR mouse Alpha-synuclein
Plasmid#89073Purposebacterial expression of mouse Alpha-synucleinDepositorAvailable SinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDule2-IBBN (G2)
Plasmid#85501PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Trigger_32B
Plasmid#75009PurposePortion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequenceDepositorInsertZIKV_Trigger_32B
ExpressionBacterialPromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K48C
Plasmid#86588PurposeBacterial expression of Ubiquitin K48C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
TagsHis6-MBPExpressionBacterialMutationTEV site; K48CPromoterTacAvailable SinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSETB-de4GFP
Plasmid#85472PurposeInducible expression of dual emitting pH sensitive GFP in bacterial cellsDepositorInsertde4GFP
Tags6xHisExpressionBacterialMutationS65T/C48S/H148C/T203CPromoterT7Available SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET41-GST-KaiC
Plasmid#68051PurposeStrain: AM5117, Used for protein purificationDepositorInsertKaiC
Tags6xHis, GST, and S-TagExpressionBacterialPromoterT7Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB078d8
Plasmid#79206PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRExpressionBacterial and InsectPromoter3XP3 eye-expression PromoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSJ111
Plasmid#78707PurposeModular vector for constitutive secreted amylase reporter expressionDepositorInsertamyEopt
ExpressionBacterialPromoterPvegAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1
Plasmid#81060Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-TIAM1 DH/PH
Plasmid#81129Purposemammalian expression of Zdk1 tagged Tiam1 Dh/PHDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD∆cat
Plasmid#81054Purposebacterial expression of catalytic dead catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057 with histid…PromoterT7Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.0
Plasmid#81050Purposebacterial expression of murine TDGDepositorAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
SIRT5H158Y-pET15b
Plasmid#81032PurposeBacterial expression of Human catalytic mutant SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
TagsHisExpressionBacterialMutationH158Y catalytic mutantPromoterT7Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only