30,261 results
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Plasmid#205086PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)
Plasmid#182395PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusionsDepositorInsertsVNp-LZ-VenusN154
VNp-LZ-VenusC155
UseTagsVesicle Nucleating peptide (VNp) & Leucine Zi…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.1.t1
Plasmid#73545PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.1.t1
pAcFRS.1.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
KG#62
Plasmid#110874PurposeExpresses the C. elegans acy-1 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PHGDH
Plasmid#74113PurposeExpression of N-terminal His-taggedDepositorInsertPHGDH (PHGDH Human)
UseTagsHis-taggedExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT_D355N
Plasmid#46988PurposeInducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
UseTagsGST and PreScission cleavage siteExpressionBacterialMutationMutation aspartic acid 355 to asparagine (D355N);…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcΔCTD
Plasmid#119878PurposeBacterial Expression construct for the production and purification of Arc missing C-terminal DomainDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
UseTagsGSTExpressionBacterialMutationDeletion of nt 832-1110PromotertacAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcNB1
Plasmid#89241PurposeResurfaced Cell-penetrating GFP nanobodyDepositorInsertpcNB1 (GFP)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAH69
Plasmid#173782PurposeThe pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.DepositorInsertHK022 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
CD Ugi3
Plasmid#177195PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 3DepositorInsertUtricularia gibba insulator 3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCellFree_G06_ZIKV E
Plasmid#145351PurposeCell-free expression vector for Zika virus Envelope protein with C-term mCherryDepositorInsertZIKV Envelope
UseTagsmCherry and 8xHisExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RhoA-T19N
Plasmid#12960DepositorInsertRhoA DN (RHOA Human)
UseTagsGSTExpressionBacterialMutationT19N Dominant negativePromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pR_Lux
Plasmid#226457Purposelambda immunity region to the luciferase operon (lux)DepositorInsertlambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-2
Plasmid#90326PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694DepositorInsertTetR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHflu4
Plasmid#203882PurposepHI1 with insertion of monomeric red fluorescent protein (mRFP)DepositorInsertmonomeric red fluorescent protein
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)
Plasmid#156463PurposeExpress Heme Oxygenase (Nostocaceae) and ReBphP-PCMDepositorInsertsHeme Oxygenase
ReBphP-PCM
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-1
Plasmid#61736PurposeStreptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNADepositorInsertssSpCas9
tracrRNA
crRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only