30,807 results

1. pDuet TDP-43 G294A

   Plasmid

   #27463

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Expression

   Bacterial

   Mutation

   Glycine 294 mutated to Alanine (G294A)

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

2. EGFP-p300 (D1399Y)

   Plasmid

   #191763

   Purpose

   Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (D1399Y) (EP300 Human)
   

   Tags

   EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

   Expression

   Bacterial and Mammalian

   Mutation

   catalytic D1399Y mutation in p300

   Promoter

   EF1alpha

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWJB9

   Plasmid

   #126650

   Purpose

   Expresses AdrA from a modified pLAFR3

   Depositor

   Ute Romling

   Article

   Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

   Insert

   adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
   

   Tags

   8xHis

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKESaPar

   Plasmid

   #211829

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

   Plasmid

   #228639

   Purpose

   Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Coxsackievirus 33 isoform B 3C protease
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDC180

   Plasmid

   #218673

   Purpose

   Expresses rat ⍺SNAP in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   rat ⍺SNAP (Napa Rat)
   

   Tags

   6 his

   Expression

   Bacterial

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a human Tankyrase2 full length Y920E

   Plasmid

   #132639

   Purpose

   expresses human Tankyrase2 full length Y920E in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

   Insert

   Tankyrase2 Y920E
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDDYFP-A

   Plasmid

   #40288

   Depositor

   Robert Campbell

   Article

   Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

   Insert

   ddYFP-A
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

9. R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

   Plasmid

   #159696

   Purpose

   E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
   

   Tags

   His6-Halotag-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJA48 HLA-B*57:01

    Plasmid

    #180444

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*57:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)

    Plasmid

    #197662

    Purpose

    Expresses GFP under the control of J23100 and RiboJ-UTR1

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_PLK1_Pkinase

    Plasmid

    #109933

    Purpose

    Protein expression and purification of PLK1_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PLK1_Pkinase
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD/His-miRFP670nano

    Plasmid

    #127427

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    araC

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Human, Synthetic)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pJL030

    Plasmid

    #53533

    Purpose

    supplies EtpC required for optimal EtpA expression on compatible plasmid

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpC
    

    Use

    Pacyc184

    Expression

    Bacterial

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBA562

    Plasmid

    #186237

    Purpose

    pTet-RfxCas13d crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-29b-3xGluGlu-c-jun-His6

    Plasmid

    #129227

    Purpose

    Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    cJun (JUN Human)
    

    Tags

    6xHistidine

    Expression

    Bacterial

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTrc99a-NoSS-TolC

    Plasmid

    #155179

    Purpose

    Expresses signal sequence-less TolC as inclusion bodies

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    TolC (tolC )
    

    Expression

    Bacterial

    Mutation

    deleted residues 1-20, residue 21 mutated to Met …

    Promoter

    trc

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFN18A-HaloTag-SpyCatcher

    Plasmid

    #206041

    Purpose

    HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Type

    Empty backbone

    Tags

    HaloTag and SpyCatcher-8His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p361_DG

    Plasmid

    #234683

    Purpose

    Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Tags

    His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

    Expression

    Bacterial

    Mutation

    No

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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