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Plasmid#177694Purposeexpresses a N-acetyl hexosamine oxidase in E. coliDepositorInsertN-acetylhexosamine oxidase
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-eGFP
Plasmid#176862PurposeExpresses Glt-1 fused with GFP at the C-terminus in astrocytesDepositorInsertGlt1-eGFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Alpha1
Plasmid#118044PurposeGoldenBraid2.0 Alpha 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLJSRSF7
Plasmid#64693PurposeHis-MBP-Smt3-NpuDnaEN102-CBDDepositorTypeEmpty backboneUseTagsCBD, HisTag, MBP, NpuDnaE-N102, and Smt3ExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DVLP1_DEP
Plasmid#109806PurposeProtein expression and purification of DVLP1_DEPDepositorInsertDVLP1_DEP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJS007
Plasmid#188330PurposePCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::wrmScarlet::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF_LuxR
Plasmid#140039PurposeConstutitive expression of LuxR protein.DepositorInsertpLuxL and LuxR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC020 - LseCas13a from Listeria seeligeri
Plasmid#91910PurposeExpresses LseCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLseCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
28 pCOLI_S_STREP
Plasmid#160262PurposeN-term 2xStrepII 3C cleavable Spec resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepIIExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HNRNPA1-Halo
Plasmid#175307PurposeProduction of HaloTag fusion proteinDepositorInsertHNRNPA1 (HNRNPA1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-Hsp90 C(626-732)
Plasmid#22483DepositorInsertHsp90alpha C domain (HSP90AA2P Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorInsertSARS-COV-2 Nsp12 (ORF1ab Synthetic)
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATLIC
Plasmid#110287PurposeA backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)DepositorInsertyfaL
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-dTomato
Plasmid#117391PurposeTn7 tagging vector pTn7xTS with dTomato expression scaffoldDepositorInsertdTomato
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-Tev-Keap1
Plasmid#62456PurposeHis6-halo tagged human Keap1 protein for E.coli expressionDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
UseTagsExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterENO1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-R-gfp
Plasmid#220927PurposeExpresses gfp using J23119 promoter and pgRNA_AT backboneDepositorInsertsuperfolder GFP
UseTagsFLAGExpressionBacterialMutationPromoterJ23119Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only