30,261 results

1. pQTEV-FLJ30046

   Plasmid

   #34828

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   FLJ30046 (SLAIN1 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSW068

   Plasmid

   #140036

   Purpose

   KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Truncated thioesterase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cLac143

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HisMS2_VLP_RSV

   Plasmid

   #175958

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

   Depositor

   Paul Freemont

   Article

   Unruh et al medRxiv 2024.04.17.24305965

   Inserts

   P gene
   M gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pET28-sfGFP-DogTag Loop A

   Plasmid

   #171779

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Val2…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1-EXO1b

   Plasmid

   #68267

   Purpose

   Bacterial expression of human EXO1

   Depositor

   Stefano Ferrari

   Article

   El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

   Insert

   EXO1 (EXO1 Human)
   

   Use

   Tags

   Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   b form, codons 1-846. Please see depositor commen…

   Promoter

   T7

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET19-MTdTwt

   Plasmid

   #207460

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTwt (Dntt Mouse)
   

   Use

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLG169

   Plasmid

   #124367

   Purpose

   Xylose inducible system with the CRE site for expression in B. subtilis

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2 (NEWENTRY Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pxyl

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAJM.884

   Plasmid

   #108536

   Purpose

   AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   AcuRAM + PAcu-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AcuR: E80K R154H L202M

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRT498-OtUBD

   Plasmid

   #190089

   Purpose

   Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBRG2

    Plasmid

    #149474

    Purpose

    Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    RhaS-RhaR-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET24c(+)-EFTu

    Plasmid

    #31172

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Tu (tufB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOP5GP

    Plasmid

    #112610

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AK1

    Plasmid

    #39134

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK1 (AK1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCVD364

    Plasmid

    #63739

    Purpose

    V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

    Depositor

    James Kaper

    Article

    Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

    Insert

    nanH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMUT2-pTcpP-mCherry

    Plasmid

    #192861

    Purpose

    pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTmpGm

    Plasmid

    #176229

    Purpose

    A template plasmid for amplification of the cI-hok-aacC4 cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, aacC4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTetO-ISce1-TetO

    Plasmid

    #58506

    Purpose

    ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Tet repeats flanking ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 MBP TEV HDAC8_374

    Plasmid

    #122174

    Purpose

    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

    Depositor

    David Christianson

    Article

    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

    Insert

    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    

    Use

    Tags

    hexahistidine tag and tobacco etch virus protease…

    Expression

    Bacterial

    Mutation

    Residues Met1–Pro7 and H375-V377 have been delete…

    Promoter

    T7

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE165

    Plasmid

    #118513

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge